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Effects of the antimicrobial contaminant triclocarban, and co-exposure with the androgen 17beta-trenbolone, on reproductive function and ovarian transcriptome of the fathead minnow (Pimephales promelas).

Metadata Updated: November 12, 2020

Triclocarban (TCC) is a widely used antimicrobial agent that is routinely detected in surface waters. The present study was designed to examine TCC’s efficacy and mode of action as a reproductive toxicant in fish. Reproductively mature Pimephales promelas were continuously exposed to either 1 or 5 μg TCC/L, 0.5 μg 17β-trenbolone (TRB)/L or a mixture (MIX) of 5 μg TCC and 0.5 μg TRB/L for 22 d and a variety of reproductive and endocrine-related endpoints were examined. The data set includes: -Concentrations of the test chemicals detected in water and tissues of exposed fish -Ex vivo production of testosterone and estradiol by gonad tissue placed in culture (ex vivo). -Plasma concentrations of testosterone, 17beta estradiol, and vitellogenin -Targeted gene expression measurements examining relative abundance of messenger RNA coding for enzymes involved in steroid synthesis: cholesterol side-chain cleavage (cyp11a), 17-a-hydroxylase/17,20 lyase (cyp17), aromatase (cyp19a1a), 3b-hydroxysteroid dehydrogenase (3bhsd), 11bhydroxysteroid dehydrogenase (11bhsd), and 17b-hydroxysteroid dehydrogenase (17bhsd) as well as five additional transcripts measured included steroidogenic acute regulatory protein (star), Vtg receptor (vtgr), follicle-stimulating hormone receptor (fshr), luteinizing hormone receptor (lhr), and androgen receptor (ar). -Ovarian transcriptomics data measured using a 15000 feature oligonucleotide microarray (GEO Platform Accession GPL10259). -Survival, reproduction, and morphological data. -.

This dataset is associated with the following publication: Villeneuve , D., K. Jensen , J. Cavallin , E. Durhan, N. Garcia-Reyero, M. Kahl , R. Leino, E. Makynen, L. Wehmas, E. Perkins, and G. Ankley. Effects of the anti-microbial contaminant triclocarban and co-exposure with the androgen 17â-trenbolone, on reproductive function and ovarian transcriptome of the fathead minnow (Pimephales promelas). ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 36(1): :231-242, (2017).

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Metadata Created Date November 12, 2020
Metadata Updated Date November 12, 2020

Metadata Source

Harvested from EPA ScienceHub

Additional Metadata

Resource Type Dataset
Metadata Created Date November 12, 2020
Metadata Updated Date November 12, 2020
Publisher U.S. EPA Office of Research and Development (ORD)
Data Last Modified 2018-10-26
Public Access Level public
Bureau Code 020:00
Schema Version
Harvest Object Id f844f31b-1f00-4e8f-8d9b-b1b231f2fcc3
Harvest Source Id 04b59eaf-ae53-4066-93db-80f2ed0df446
Harvest Source Title EPA ScienceHub
Program Code 020:095
Publisher Hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
Related Documents,
Source Datajson Identifier True
Source Hash e8f96d42982e01a3524de9c4e3133cf7b901e076
Source Schema Version 1.1

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