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Data and code from: Use of automated capillary immunoassay for quantification of antibodies in chicken sera against recombinant Salmonella enterica serotype Heidelberg proteins

Metadata Updated: August 2, 2024

This entry contains all data and code required to reproduce the analysis of immune response data presented in the manuscript: Yeh, H.-Y., J. G. Frye, C. R. Jackson, Q. D. Read, J. E. Line, and A. Hinton. 2023. Use of automated capillary immunoassay for quantification of antibodies in chicken sera against recombinant Salmonella enterica serotype Heidelberg proteins. Journal of Microbiological Methods. https://doi.org/10.1016/j.mimet.2023.106757 Files included are:

chicken_salmonella_immunoassay_analysis.Rmd: RMarkdown notebook with all analysis code and full supplementary results, figures, tables, and explanatory notes. The Excel file containing the data should be in the same dictionary as the .Rmd file when the notebook is rendered. chicken_salmonella_immunoassay_analysis.html: rendered output of notebook including full supplementary results, figures, and tables, and explanatory text. Chicken Sera FliD FimA.xlsx: Excel file with chicken immune response data in the form of chemiluminescence values (arbitrary units) that are a proxy for immunoglobulin concentration. Each row contains measurements from an individual bird, across two time points (bleedings); at each time point IgG chemiluminescence values in response to Salmonella FliD and FimA proteins are given, and IgM chemiluminescence as well. This results in a total of eight measurements per bird (2 bleedings x 2 types of immunoglobulin x 2 Salmonella proteins). The first ten rows are non-immunized control birds and the next ten rows are immunized birds. concentration_chemiluminescence.xlsx: Excel file with protein concentration and chemiluminescence data to verify relationship between them. There are three columns, one to identify the protein (FliD and FimA), one for known protein concentration, and one for chemiluminescence. This is used to verify that the chemiluminescence values are suitable proxies for protein concentration.

This research is associated with USDA project 6040-32000-079-00D and falls under National Program NP108. Resources in this dataset:Resource Title: RMarkdown document with immune response model fitting code. File Name: chicken_salmonella_immunoassay_analysis.RmdResource Title: Rendered HTML version of notebook. File Name: chicken_salmonella_immunoassay_analysis.htmlResource Title: Immunoglobulin response data. File Name: Chicken Sera FliD FimA.xlsxResource Title: Concentration and chemiluminescence data. File Name: concentration_chemiluminescence.xlsx

Access & Use Information

Public: This dataset is intended for public access and use. License: us-pd

Downloads & Resources

Dates

Metadata Created Date March 30, 2024
Metadata Updated Date August 2, 2024

Metadata Source

Harvested from USDA JSON

Additional Metadata

Resource Type Dataset
Metadata Created Date March 30, 2024
Metadata Updated Date August 2, 2024
Publisher Agricultural Research Service
Maintainer
Identifier 10.15482/USDA.ADC/1528601
Data Last Modified 2024-07-09
Public Access Level public
Bureau Code 005:18
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id 9dd2ce14-fb9e-45f2-acd2-e3aabf89b86c
Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
Harvest Source Title USDA JSON
License https://www.usa.gov/publicdomain/label/1.0/
Old Spatial {"type": "Point", "coordinates": -83.359019076718, 33.925811652341}
Program Code 005:040
Source Datajson Identifier True
Source Hash e95e162e03a7193c4b94c13a2b2629d7d574334509c81842324c1c87143a1f80
Source Schema Version 1.1
Spatial {"type": "Point", "coordinates": -83.359019076718, 33.925811652341}

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