These data represent temporal changes in the concentrations of individual oxylipins (OxL) and endocannabinoids (eCB) in adult women during a sub-maximal aerobic exercise bout, and at recovery (20 min. post-cessation of exercise) (Supplementary Materials 1). Results are depicted both before (pre-) and after (post-) a weight loss and fitness intervention lasting at least 14 wk. Participants were obese, sedentary, and insulin resistant in the pre-intervention phase. Also depicted are statistical groupings of metabolites, as an Excel file (Supplementary Materials 2). Resources in this dataset:Resource Title: Supporting Materials 1. File Name: oxy_timecourse, SUPPLEMENTAL MATERIALS 1, for submission.pdfResource Description: Blood plasma oxylipin and endocannabinoid concentrations over time during an acute sub-maximal aerobic exercise bout in women, both before and after a 14 wk+ fitness and weight loss intervention. Total exercise time = 30 min, followed by a recovery period of 20 min.Resource Software Recommended: Adobe Acrobat,url: https://acrobat.adobe.com/us/en/acrobat/pdf-reader.html Resource Title: Supporting Materials 2, oxylipin and endocannabinoid statistics & clustering. File Name: SUPPLEMENTAL MATERIALS 2, for submissionXLS.xlsResource Description: Excel sheet with statistics and statistical pattern clustering associated with blood plasma oxylipins and endocannabinoids in women performing 30 min. sub-maximal aerobic exercise, followed by a 20 min recovery period.Resource Software Recommended: Microsoft Excel,url: https://products.office.com/en-us/excel