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Data from: Transcriptomes of bovine ovarian follicular and luteal cells

Metadata Updated: March 30, 2024

Affymetrix Bovine GeneChip® Gene 1.0 ST Array RNA expression analysis was performed on four somatic ovarian cell types: the granulosa cells (GCs) and theca cells (TCs) of the dominant follicle and the large luteal cells (LLCs) and small luteal cells (SLCs) of the corpus luteum. The normalized linear microarray data was deposited to the NCBI GEO repository (GSE83524). Subsequent ANOVA determined genes that were enriched (≥2 fold more) or decreased (≤−2 fold less) in one cell type compared to all three other cell types, and these analyzed and filtered datasets are presented as tables. Genes that were shared in enriched expression in both follicular cell types (GCs and TCs) or in both luteal cells types (LLCs and SLCs) are also reported in tables. The standard deviation of the analyzed array data in relation to the log of the expression values is shown as a figure. These data have been further analyzed and interpreted in the companion article "Gene expression profiling of ovarian follicular and luteal cells provides insight into cellular identities and functions", Romereim et al., (2017) Mol. Cell. Endocrinol. 439:379-394. https://doi.org/10.1016/j.mce.2016.09.029 Resources in this dataset:Resource Title: RNA Expression Data from Four Isolated Bovine Ovarian Somatic Cell Types. File Name: Web Page, url: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE83524 NCBI Gene Expression Omnibus (GEO) Accession Display. Analysis of the RNA present in each bovine cell type using Affymetrix microarrays yielded new cell-specific genetic markers, functional insight into the behavior of each cell type via Gene Ontology Annotations and Ingenuity Pathway Analysis, and evidence of small and large luteal cell lineages using Principle Component Analysis. Enriched expression of select genes for each cell type was validated by qPCR. This expression analysis offers insight into the lineage and differentiation process that transforms somatic follicular cells into luteal cells. The orignal Affymetrix .CEL files and the normalized linear expression data are included in this submission.

Access & Use Information

Public: This dataset is intended for public access and use. License: Creative Commons Attribution

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Dates

Metadata Created Date March 30, 2024
Metadata Updated Date March 30, 2024

Metadata Source

Harvested from USDA JSON

Additional Metadata

Resource Type Dataset
Metadata Created Date March 30, 2024
Metadata Updated Date March 30, 2024
Publisher Agricultural Research Service
Maintainer
Identifier 10.1016/j.dib.2016.11.093
Data Last Modified 2024-02-09
Public Access Level public
Bureau Code 005:18
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id 06613a04-f4eb-47bd-b25f-89ccf8a81766
Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
Harvest Source Title USDA JSON
License https://creativecommons.org/licenses/by/4.0/
Program Code 005:040
Source Datajson Identifier True
Source Hash 005f2309bd706c606ce115b7c364a26517a8f81a0d02a5562aff0d3a9740b086
Source Schema Version 1.1

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