Switchgrass (Panicum virgatum L.) is a warm-season grass native to the tallgrass prairie in North America with various ecosystem benefits and enhance the resiliency of ecosystems. These resilient qualities of switchgrass may be due to the steroidal saponins produced by the grass. Steroidal saponins are plant secondary metabolites in the group of terpenes. Steroidal saponins are triterpenes that have antibacterial and antifungal characteristics. Switchgrass cultivars “Liberty” and “Independence” are two prominent bioenergy switchgrass cultivars for the Great Plains and Midwest. This dataset shows the relative steroidal saponin concentration in leaf and stem tissues from both Liberty and Independence switchgrass. The steroidal saponins, protodioscin, dichotomin, and saponin B, were detected in all leaf and stem tissues of both Liberty and Independence cultivars.