This dataset was generated from soybean (Glycine max) field trials conducted at the West Tennessee Research and Education Center in Jackson, TN and at the Research and Education Center at Milan in Milan, TN as well as from molecular marker screening conducted at the West Tennessee Research and Education Center in Jackson, TN. Table 3 includes measured data for height, yield, and seed size, and rating data for lodging and seed quality for JTN-5110, 5601T, and select other released germplasm lines and cultivars tested in replicated breeder yield trials in Jackson and Milan, TN from 2010-2016, excluding 2014. This data may be useful in measuring yield gain in future releases of soybean germplasm or cultivars with broad resistance to soybean cyst nematode (SCN; Heterodera glycines). This data should not be used to measure yield gain for elite high-yielding cultivars that do not have broad cyst nematode resistance. Table 5 includes rating data for JTN-5110 and soybeans with established SCN resistance from simple sequence repeat (SSR) markers: Satt309 and Sat_168, associated with rhg1 on chromosome 18; Sat_162, associated with Rhg4 on chromosome 8; and Satt574, associated with cqSCN-005 on chromosome 17. This data may be useful in understanding the role of these molecular regions in SCN resistance for JTN-5110 and parent line Anand. This data should not be used to draw broad conclusions about cyst nematode resistance, in general. Table 7 includes rating data for JTN-5110 and check cultivars from frogeye leafspot (caused by Cercospora sojina) field disease screenings conducted in Milan, TN from 2010-2012. This data may be useful in measuring changes in frogeye leafspot incidence and severity in West Tennessee. This data should not be used to draw broad conclusions or represent different geographic areas. Resources in this dataset:Resource Title: Data dictionary. File Name: data dictionary.csvResource Description: A data dictionary defining the fields in Tables 3, 5, and 7Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel Resource Title: Table 3 - JTN-5110 compared to 5601T. File Name: Table 3 - JTN-5110 compared to 5601T.csvResource Description: Breeder yield trial data from Jackson and Milan, TN from 2010-2016, excluding 2014Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel Resource Title: Table 5 - compiled marker data. File Name: Table 5 - compiled marker data.csvResource Description: Genetic marker data for SSR markers associated with soybean cyst nematode resistance. Screening conducted in Jackson, TN from 2005-2020.Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel Resource Title: Table 7 - frogeye leafspot evaluation. File Name: Table 7 - frogeye leafspot evaluation.csvResource Description: Data from frogeye leafspot field screening conducted in Milan, TN from 2010-2012.Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel