Data and supplemental figures for manuscript titled "Net release and uptake of xenometabolites across intestinal, hepatic, muscle, and renal tissue beds in healthy conscious pigs." We used a catheterized conscious pig model to estimate inter-organ flux of xenometabolites, derivatives, and bile acids using LC/MS. Female pigs (n=12; 2-3 months old; 25.6 ± 2.2 kg) had surgically-implanted catheters across portal-drained viscera (PDV), splanchnic area (SPL), liver, kidney, and hindquarter muscle. Overnight fasted arterial and venous plasma was collected simultaneously in a conscious state and stored at -80°C. Thawed samples were analyzed by liquid chromatography-mass spectrometry. Plasma flow was determined with para-aminohippuric acid dilution technology and used to calculate net organ balance for each metabolite. This repository contains the LC/MS data and supplemental figures for the submitted manuscript. Resources in this dataset:Resource Title: Organ Net Balance Data for Mercer et al.. File Name: Mercer et al LCMS XM Organ Flux Data.xlsxResource Description: Calculated net balances for LC/MS data from Mercer et al. Resource Title: LC/MS data for Mercer et al.. File Name: Mercer et al LCMS XM raw data.xlsxResource Description: LC/MS peak area data for xenometabolites in Mercer et al.Resource Title: Mercer et al Supplemental Figure 2. File Name: Mercer et al Fig S2.pdfResource Description: Supplemental Figure 2 for Mercer et al manuscript: Absolute differences of xenometabolites from catheter ports in conscious overnight-fasted female pigs (n=12). Sampling sites included arterial, venous, renal, portal, and hepatic placed catheters. Resource Title: Mercer et al Supplemental Figure 1. File Name: Mercer et al Fig S1.pdfResource Description: Supplemental Figure 1 for Mercer et al manuscript: Supplemental Figure 1: Inter-organ flux of all xenometablites in conscious overnight-fasted female pigs (n=12). A negative flux reflects net uptake (flux 0; indicated by top arrow) by the organ. Plasma flow measurements were determined with the para-aminohippuric acid dilution method, and were used along with metabolite concentrations to calculate flux (see Methods). R at top of arrow indicates ‘Release’, while U indicates ‘Uptake’. Resource Title: Supplemental Table 1 for Mercer et al.. File Name: Mercer et al Table S1.xlsxResource Description: Mercer et al Supplemental Table 1: Raw differences of identified xenometabolites from port samples in conscious adult female pigs (n=10-12).