The ascomycete fungus Cryphonectria parasitica is the causal agent of chestnut blight disease. This deadly fungal pathogen was introduced into North America from Asia before the turn of the 20th century, quickly spreading throughout the natural range of the American chestnut tree. In the course of a single generation, chestnut blight destroyed billions of American chestnut trees in forests across North America, driving it almost to extinction. The genome assembly for C. parasitica EP155 (v. 2.0, available at https://mycocosm.jgi.doe.gov/Crypa2/Crypa2.info.html) contains 26 main genome scaffolds totaling 43.9 Mb, and was sequenced at the U.S. Department of Energy Joint Genome Institute. The information and documents contained within this Ag Data Commons dataset provide supplementary data about the EP155 genome assembly, including scaffold summaries, genetic maps, mitochondrial DNA, P450s, secondary metabolite clusters, vegetative incompatibility genes, and transposable elements. These data are freely available for research purposes. Resources in this dataset:Resource Title: Secondary metabolite summary. File Name: EP155 secondary metabolits.xlsxResource Description: Predicted secondary metabolites in the genome assembly of Cryphonectria parasitica EP155.Resource Title: Cryphonectria parasitica EP155 Genetic Maps. File Name: Genome Fig. 1_120611.pptxResource Description: Alignment of Cryphonectria parasitica EP155 V2.0 genome scaffolds 1 and 2 with the genetic linkage map for cross MJ1 (JA17 × X17-8) (Kubisiak and Milgroom 2005). Resource Title: Phylogenetic tree of the cytochrome P450 proteins (P450ome) in Cryphonectria parasitica EP155. . File Name: Figure_P450_EP155.pptxResource Title: Family/subfamily-level classification of the cytochrome P450 monooxygenases (P450ome) of Cryphonectria parasitica EP155.. File Name: Crypa2-Supplementary Tables.xlsxResource Title: Summary of scaffold sizes in the Cryphonectria parasitica EP155 v.2.0 genome assembly.. File Name: scaffolds.xlsxResource Title: Cryphonectria parasitica EP155 unmasked nuclear genome assembly. File Name: Cryphonectriaparasiticav2.nuclearAssembly.unmasked.txtResource Title: Cryphonectria parasitica EP155 gene catalog. File Name: Cparasiticav2.GeneCatalog20091217.proteins.txtResource Title: Cryphonectria parasitica EP155 mitochondial genome assembly. File Name: EP155mitochondria.fasta.txt