This article contains consolidated proteomic data obtained from xylem sap collected from tomato plants grown in Fe- and Mn-sufficient control, as well as Fe-deficient and Mn-deficient conditions. Data presented here cover proteins identified and quantified by shotgun proteomics and Progenesis LC-MS analyses: proteins identified with at least two peptides and showing changes statistically significant (ANOVA; p ≤ 0.05) and above a biologically relevant selected threshold (fold ≥ 2) between treatments are listed. The comparison between Fe-deficient, Mn-deficient and control xylem sap samples using a multivariate statistical data analysis (Principal Component Analysis, PCA) is also included. Data included in this article are discussed in depth in "Effects of Fe and Mn deficiencies on the protein profiles of tomato (Solanum lycopersicum) xylem sap as revealed by shotgun analyses", Ceballos-Laita et al., J. Proteomics, 2018. This dataset is made available to support the cited study as well to extend analyses at a later stage. Resources in this dataset:Resource Title: ProteomeExchange submission PXD007517. Xylem sap shotgun proteomics from Fe- and Mn-deficient and Mn-toxic tomato plants. . File Name: Web Page, url: http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD007517 The MS proteomics data have been deposited to the ProteomeXchange Consortium via the Pride partner repository with the data set identifier PXD007517. Also includes FTP location. Files available at https://www.ebi.ac.uk/pride/archive/projects/PXD007517 via HTML, FTP, or Fast (Aspera) download : 1 SEARCH.xml file, 1 Peak file, 24 RAW files, 1 Mascot information.xlsx file. Supplementary data associated with this article can be found in the online version at http://dx.doi.org/10.1016/j.dib.2018.01.034