Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Skip to content
  • Feedback

    • CottonGen Primer3

    Metadata Updated: April 21, 2025

    Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:

    oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,

    PCR product size,

    positional constraints within the source (template) sequence, and

    possibilities for ectopic priming (amplifying the wrong sequence)

    many other constraints.

    All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair. Resources in this dataset:Resource Title: Website Pointer for CottonGen Primer3. File Name: Web Page, url: https://www.cottongen.org/tools/primer3

    Access & Use Information

    Public: This dataset is intended for public access and use. License: us-pd

    Downloads & Resources

    Dates

    Metadata Created Date March 30, 2024
    Metadata Updated Date April 21, 2025

    Metadata Source

    Harvested from USDA JSON

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date March 30, 2024
    Metadata Updated Date April 21, 2025
    Publisher Agricultural Research Service
    Maintainer
    Identifier 10113/AA23039
    Data Last Modified 2024-02-13
    Public Access Level public
    Bureau Code 005:18
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 06fc41cf-2786-4a94-9759-c4f5a36ba490
    Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
    Harvest Source Title USDA JSON
    License https://www.usa.gov/publicdomain/label/1.0/
    Program Code 005:040
    Source Datajson Identifier True
    Source Hash b0b328502806333a0a370853bc3f9b9b1c1d001af9af4e539dc2223eadfdd8e3
    Source Schema Version 1.1

    Didn't find what you're looking for? Suggest a dataset here.