This data set describes experiments that were conducted to investigate whether exposure of male fathead minnows to environmentally-relevant estrone concentrations would result in elevated plasma 17β-estradiol concentrations in the fish. Secondly, we sought to establish whether observed elevations in plasma 17β-estradiol occurred as a result of the conversion of external estrone by the fish using an approach involving exposure of the fish to 13C-labeled estrone. Endpoints reported in the dataset include plasma 17β-estradiol and estrone concentrations, plasma vitellogenin concentrations, hepatic vitellogenin mRNA, 13C-labeled plasma 17β-estradiol and estrone concentrations, and testicular and/or hepatic expression of aromatase and several hydroxysteroid dehydrogenases involved in estrone metabolism.

This dataset is associated with the following publication: Ankley, G., D. Feifarek, B. Blackwell, J. Cavallin, K. Jensen, M. Kahl, S. Poole, E. Randolph, T. Saari, and D. Villeneuve. Reevaluating the significance of estrone as an environmental estrogen (article). ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 51: 4705-4713, (2017).