Regional epithelial cell diversity in the small intestine of pigs

Single cell suspensions enriched for epithelial cells were obtained from duodenum, jejunum, and ileum of a 7.5-week-old pig and subjected to single-cell RNA sequencing (scRNA-seq). scRNA-seq was performed to provide transcriptomic profiles of epithelial cells, with 695 cells annotated into 6 cell types. Deeper interrogation of data revealed previously undescribed cells in porcine intestine, and region-specific gene expression profiles within specific cell subsets. Data herein includes a .h5seurat files of the epithelial cell subsets analyzed. Files may be used to reconstruct different analyses and perform further data query. Scripts for original data analyses are found at https://github.com/USDA-FSEPRU/scRNAseqEpSI_Pilot. Raw data are available at GEO accession GSE208613. Data are available for online query at https://singlecell.broadinstitute.org/single_cell/study/SCP1936/regional-epithelial-cell-diversity-in-the-small-intestine-of-pigs. Resources in this dataset:Resource Title: .h5Seurat object - epithelial cells. File Name: EpithelialCells.tarResource Description: Epithelial cells used for data analysis, available in .h5Seurat file format. Untar file before use.

[
  "005:18"
]

{
  "fn": "Loving, Crystal",
  "hasEmail": "mailto:crystal.loving@usda.gov"
}

[
  {
    "@type": "dcat:Distribution",
    "title": "EpithelialCells.tar",
    "format": "tar",
    "mediaType": "application/octet-stream",
    "downloadURL": "https://ndownloader.figshare.com/files/44532434"
  }
]

[
  "ARS",
  "BEST4",
  "NP108",
  "Pig",
  "data.gov",
  "enterochromaffin",
  "enterocyte",
  "enteroendocrine",
  "epithelial",
  "epithelium",
  "intestine",
  "scRNA-seq",
  "single-cell",
  "single-cell RNA sequencing"
]

[
  "005:040"
]

{
  "name": "Agricultural Research Service",
  "@type": "org:Organization"
}

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