{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["005:18"], "contactPoint": {"fn": "Loving, Crystal", "hasEmail": "mailto:crystal.loving@usda.gov"}, "description": "<p>Single cell suspensions enriched for epithelial cells were obtained from duodenum, jejunum, and ileum of a 7.5-week-old pig and subjected to single-cell RNA sequencing (scRNA-seq). scRNA-seq was performed to provide transcriptomic profiles of epithelial cells, with 695 cells annotated into 6 cell types. Deeper interrogation of data revealed previously undescribed cells in porcine intestine, and region-specific gene expression profiles within specific cell subsets. Data herein includes a .h5seurat files of the epithelial cell subsets analyzed. Files may be used to reconstruct different analyses and perform further data query. Scripts for original data analyses are found at <a href=\"https://github.com/USDA-FSEPRU/scRNAseqEpSI_Pilot\">https://github.com/USDA-FSEPRU/scRNAseqEpSI_Pilot</a>. Raw data are available at GEO accession GSE208613. Data are available for online query at <a href=\"https://singlecell.broadinstitute.org/single_cell/study/SCP1936/regional-epithelial-cell-diversity-in-the-small-intestine-of-pigs\">https://singlecell.broadinstitute.org/single_cell/study/SCP1936/regional-epithelial-cell-diversity-in-the-small-intestine-of-pigs</a>. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: .h5Seurat object - epithelial cells.</p> <p>File Name: EpithelialCells.tar</p><p>Resource Description: Epithelial cells used for data analysis, available in .h5Seurat file format. Untar file before use.</p></li></ul><p></p>", "distribution": [{"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/44532434", "format": "tar", "mediaType": "application/octet-stream", "title": "EpithelialCells.tar"}], "identifier": "10.15482/USDA.ADC/1527979", "keyword": ["ARS", "BEST4", "NP108", "Pig", "data.gov", "enterochromaffin", "enterocyte", "enteroendocrine", "epithelial", "epithelium", "intestine", "scRNA-seq", "single-cell", "single-cell RNA sequencing"], "license": "https://creativecommons.org/licenses/by-sa/4.0/", "modified": "2025-11-21", "programCode": ["005:040"], "publisher": {"@type": "org:Organization", "name": "Agricultural Research Service"}, "spatial": "{\"type\": \"Polygon\", \"coordinates\": [[[-93.581439973495, 42.034851789973], [-93.581439973495, 42.053922832501], [-93.569766999863, 42.053922832501], [-93.569766999863, 42.034851789973], [-93.581439973495, 42.034851789973]]]}", "temporal": "2019-09-26/2019-09-26", "title": "Regional epithelial cell diversity in the small intestine of pigs"}