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Data from: The food additive butylated hydroxyanisole minimally affects the human gut microbiome ex vivo

Published by Agricultural Research Service | Department of Agriculture | Catalog Last Checked: August 03, 2026 at 05:01 PM | Dataset Last Updated: June 11, 2026
An ex vivo study of the effects on the human gut microbiota of the food additive butylated hydroxyanisole (BHA) at a concentration of 0.35 g/L. Incubations were performed on fecal slurries from 24 donors spanning 3 age groups (toddler, adults 25-40 years, adults 60+ years). Samples include initial time point (pre-incubation) and untreated controls and treated samples after 24 hours of incubation. Data collected include: shotgun metagenomic DNA sequencing, biomass (cell counts), short-chain fatty acid concentrations, gas and pH. These measures and donor metadata are in this dataset with sample IDs linking to sequencing data housed in the NCBI Sequence Read Archive associated with BioProject PRJNA1160256.

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