USDA-ARS coordinated regional wheat (Triticum aestivum L.) breeding trials examine agronomic performance and adaptation over a wider geographic range than single breeding programs can achieve. The trials provide an evaluation of experimental breeding lines in alternate test sites that are environmentally similar or dissimilar to the program of origin. Data from USDA-ARS Hard Winter Wheat Regional Nurseries grown in 1987 to 2014 were used to identify similarities among Great Plains test sites. Mean correlations of entry grain yields across locations and years were used in principal factor analyses to cluster them into production zones. The procedures used were identical to those of a previously published analysis using test data from 1959 to 1989. Five factors explained 67% of the variance in the correlation matrix among Southern Regional Performance Nursery (SRPN) locations. The analysis divided the SRPN into four major Great Plains production zones, designated Southeast, Northwest, Southwest and Northeast. The remaining minor production zone consisted of only two central South Dakota locations, both outside the typical target area and selection site of SRPN entries. In the Northern Regional Performance Nursery (NRPN), five production zones were established, with location separation predominantly resulting from east–west differences in performance. The SRPN and NRPN wheat production zones closely follow previously described ecological zones of adaptation of native Great Plains plant species. Wheat breeding programs and growers may continue to use the production zones established via the USDA-ARS coordinated winter wheat regional nurseries to target and select germplasm for crossing and for production.