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    • Data from: Rapid and cost-effective screening of genetic markers associated with pyrethroid resistance in <i>Musca domestica</i> using RNAse H2 PCR (rhPCR)

    Metadata Updated: June 5, 2025

    Pyrethroid resistance, particularly knockdown resistance (kdr), is widespread in insect pest populations, but rarely has kdr been associated with field-level pest control failure. The prevailing understanding is that kdr contributes to a resistant phenotype, but this knowledge has remained largely an academic pursuit and has not translated to tools and strategies needed by agricultural producers to make rapid decisions for effective resistance management. As a first step in providing these operational tools, we developed robust assays using the high specificity of rhPCR to reduce kdr assessment time by approximately 80% and costs ~75% from the traditional Sanger based method used for Musca domestica. An important consideration for the use of an operational tool is the ability to get an accurate result on the first attempt, so we used Nanopore sequencing to confirm genotypes in a subset of samples and found the first pass genotyping accuracy of rhPCR method to be 75.0%, versus 41.2% with the traditional Sanger method. To demonstrate the broad applicability and comparability of screening for kdr SNPs using rhPCR, we conducted the largest assessment of kdr genotypes of M. domestica in United States dairy operations and found similar kdr patterns to other recent studies using traditional methods.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: Creative Commons CCZero

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    Dates

    Metadata Created Date November 2, 2024
    Metadata Updated Date June 5, 2025

    Metadata Source

    Harvested from USDA JSON

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date November 2, 2024
    Metadata Updated Date June 5, 2025
    Publisher Agricultural Research Service
    Maintainer
    Identifier 10.15482/USDA.ADC/25044329.v1
    Data Last Modified 2025-05-06
    Public Access Level public
    Bureau Code 005:18
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 2cf1c749-7b51-4ae7-a5dd-6542ec045627
    Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
    Harvest Source Title USDA JSON
    License https://creativecommons.org/publicdomain/zero/1.0/
    Program Code 005:040
    Source Datajson Identifier True
    Source Hash e4f2a62607fd187f1ff4b7e12c99e157c0c6434cc0fdfe8932cc38556b33713a
    Source Schema Version 1.1
    Temporal 2021-12-01/2021-12-01

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