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Data from: Prolonged Diapause in Mormon Crickets: Embryonic Responses to Three Measures of Time

Metadata Updated: August 2, 2024

Mormon cricket eggs can remain diapausing in soil for multiple years without forming an embryo. I investigated whether embryonic development was dependent on the number of annual cycles since the egg was laid, duration of the summer period (forcing), or duration of the winter period (chilling). This was a lab experiment conducted at the ARS Northern Plains Agricultural Laboratory in Sidney Montana on two populations: Arizona (AZ, 36.5018 N, 112.1405 W, 2592 m above sea level) and Wyoming (WY, 44.4644 N, 107.4592 W, 2653 m a.s.l). The timing of Mormon cricket egg development was measured for each sibling group (designated mating pairs in the header row) within a population. Hence eggs within sibling groups are considered independent observations, and each sibling group was ultimately analyzed separately. Each sibling group was split into three treatments: 2cycle, 3cycle, and 4cycle (designated 2, 3, and 4 in the data), named for the number of seasonal cycles completed within a 60 week period. Two-cycle is best described as 15 weeks of winter, 3 weeks of spring, 10 weeks of summer, and 2 weeks of autumn; three-cycle is 6 weeks of winter, 2 weeks of spring, 10 weeks of summer, and 2 weeks of autumn; and four-cycle is 6 weeks of winter, 2 weeks of spring, and 5 weeks of summer, and 2 weeks of autumn. For 2cycle and 3cycle, eggs were screened for development in the final two weeks of each cycle (the autumn period), and the developed eggs were separated from the undeveloped eggs. Half-developed eggs (or ‘middies’) were also separated from the undeveloped eggs to follow the completion of their development separately. Initially, eggs in 4cycle were also screened in each ‘autumn’ period, but the embryos were unable to complete development in a single screening. Therefore, we were able to screen 4cycle every other autumn period. For each egg in the sibling group, we tracked the timing of the fate of each egg: either fully developed (and thus ready to enter winter and hatch the following spring), discard (due to being flat, black, or invaded by fungus), broken, mia (or missing in action when accidentally lost), or right censored (for the few eggs in a sibling group that still remained undeveloped at the end of the experiment). Any middies that were found hatched were assumed to have completed development in the prior summer. For some mating pairs, we also collected a few eggs and fixed them, which killed the eggs, but cleared the chorion so that we could review their developmental stages (fate=fixed). The treatments were applied for approximately 10 calendar years starting in 2013 when the eggs were collected and ending in 2023. The timing of the fate of each egg was calculated in three ways: 1) the number of warm periods that the egg had experienced (designated ‘wp’ in the header row), the number of weeks of chill time that the egg had experienced prior to its fate (designated ‘winter chill’), and the number of weeks of warm period that the egg had experienced when its fate was determined (designated ‘summer weeks’ although it also includes the 2 weeks of autumn).

Access & Use Information

Public: This dataset is intended for public access and use. License: us-pd

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Dates

Metadata Created Date April 10, 2024
Metadata Updated Date August 2, 2024
Data Update Frequency irregular

Metadata Source

Harvested from USDA JSON

Additional Metadata

Resource Type Dataset
Metadata Created Date April 10, 2024
Metadata Updated Date August 2, 2024
Publisher Agricultural Research Service
Maintainer
Identifier 10.15482/USDA.ADC/25219727.v1
Data Last Modified 2024-07-19
Public Access Level public
Data Update Frequency irregular
Bureau Code 005:18
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id b119252b-6afc-4bc2-a5d9-1ceb60929cc2
Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
Harvest Source Title USDA JSON
License https://www.usa.gov/publicdomain/label/1.0/
Old Spatial {"type": "MultiPoint", "coordinates": -112.1405, 36.5018, -107.4592, 44.4644}
Program Code 005:040
Source Datajson Identifier True
Source Hash 41148c61829c8821f9c27b66a4c1d5c756f7124c30f895abe5884d33134eb5fd
Source Schema Version 1.1
Spatial {"type": "MultiPoint", "coordinates": -112.1405, 36.5018, -107.4592, 44.4644}

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