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    • Data from: Impact of <i>Aedes aegypti</i> V1016I and F1534C knockdown resistance genotypes on operational interventions

    Metadata Updated: October 2, 2025

    Resistance to pyrethroids is common in Aedes aegypti populations. Mutations in the voltage gated sodium channel have an influence on the insecticide resistance (IR) phenotype. In much of the Western hemisphere, two of these knockdown resistance (kdr) mutations, V1016I and F1534C, are widely found and result in six commonly found kdr genotype combinations in field populations. Strong pyrethroid IR and the failure of permethrin treated uniforms have been linked to the presence of the homozygous double kdr genotype (IICC) but the effects of these various kdr combinations have not been rigorously examined. To better understand the impacts of these mutations, we isolated three kdr haplotypes (VF, VC, & IC) from a field collected strain to produce six Ae. aegypti isoline strains with all common kdr genotypes. We then characterized the effects of these kdr genotypes by CDC bottle bioassay and topical application and found increasing resistance to permethrin and deltamethrin as the number of IC haplotypes increased. Neither enzymatic activity nor malathion IR increased as pyrethroid IR increased. Field and wind tunnel spray of a pyrethrin formulation showed that even moderate resistance could significantly reduce knockdown and mortality. Studies with a synergized pyrethroid formulation showed effective recovery of mortality against all genotypes except for the IICC. In human bite studies, one or two IC haplotypes compromised the efficacy of permethrin treated fabrics. This study demonstrates that kdr mutations have distinct phenotypic effects in both the laboratory and operational interventions, and that the strength of pyrethroid resistance is linked to the number of IC haplotypes present in the kdr gene. Assessing kdr genotype is therefore critical for understanding target-site-mediated IR in Ae. aegypti.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: Creative Commons CCZero

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    Dates

    Metadata Created Date November 2, 2024
    Metadata Updated Date October 2, 2025

    Metadata Source

    Harvested from USDA JSON

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date November 2, 2024
    Metadata Updated Date October 2, 2025
    Publisher Agricultural Research Service
    Maintainer
    Identifier 10.15482/USDA.ADC/25802143.v1
    Data Last Modified 2025-09-24
    Public Access Level public
    Bureau Code 005:18
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id e872df62-46bc-450c-a2a4-34be6432b2b6
    Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
    Harvest Source Title USDA JSON
    License https://creativecommons.org/publicdomain/zero/1.0/
    Program Code 005:040
    Source Datajson Identifier True
    Source Hash b9c02951094b2e6c38ce424b821a365966bbb2548e7fb5b0ca3790e76831a0af
    Source Schema Version 1.1
    Temporal 2017-06-01/2017-06-01

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