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Data from: Exposure of dengue-1 virus to Aedes aegypti and sensitivity to insecticides

Metadata Updated: June 5, 2025

"PhD obj3 LD50 data submitted April 21 2025": applied to Table 1 of manuscript; used to calculate the lethal dose at which 25%, 50%, and 90% of the organisms (Aedes aegypti) die (LD25, LD50, LD90) using Probit software; PoloPlus - Leora Software. These data were generated by RLA under the supervision of KJL in July-August, 2021, at the USDA-ARS Center for Medical, Agricultural and Veterinary Entomology (USDA-ARS-CMAVE) facility in Gainesville, FL. using dilutions of technical malathion, technical permethrin, and rapeseed methyl ester. These values were generated in order to identify an LD50 dose to apply to 17d old female Ae. aegypti (7 days old prior to blood meal), 10 days after feeding on a blood meal containing dengue-1 virus. Ae. aegypti to determine the LD50 were blood fed at 7 d old, and aged 10 d after blood feeding, then exposed to the dilutions of technical malathion, technical permethrin, and rapeseed methyl ester."PhD Obj3 dengue mortality check submitted April 21 2025": used to populate the results for Table 2 and Table 3; describing the differences in mortality associated with exposure to a dengue-1 treated bloodmeal and LD50 pesticide/control treatment exposure in Aedes aegypti mosquitoes. Analysis was conducted using R. These data were generated by RLA under the supervision of BWA at the University of Florida - Florida Medical Entomology Laboratory (UF-FMEL) facility in Vero Beach, FL between September and November 2021. Ae. aegypti females were aged to 7 d, provided a dengue-1 tainted blood meal or sham blood meal, allowed to age for 10 d after blood feeding, then a topical LD50 dose of technical permethrin, technical malathion diluted in rapeseed methyl ester or neat rapeseed methyl ester as a control were applied and mortality was recorded at 24 h and 48 h. Following mortality checks, Ae. aegypti were frozen (-80 deg C) and checked for the presence of virus using RT-qPCR.

Access & Use Information

Public: This dataset is intended for public access and use. License: Creative Commons CCZero

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Dates

Metadata Created Date June 5, 2025
Metadata Updated Date June 5, 2025
Data Update Frequency R/P1M

Metadata Source

Harvested from USDA JSON

Additional Metadata

Resource Type Dataset
Metadata Created Date June 5, 2025
Metadata Updated Date June 5, 2025
Publisher Agricultural Research Service
Maintainer
Identifier 10.15482/USDA.ADC/28836149.v1
Data Last Modified 2025-05-06
Public Access Level public
Data Update Frequency R/P1M
Bureau Code 005:18
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id 98054020-6cf4-4a36-93a0-e34ae1384058
Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
Harvest Source Title USDA JSON
License https://creativecommons.org/publicdomain/zero/1.0/
Program Code 005:040
Source Datajson Identifier True
Source Hash 819ffb356eaba66d87830627d209247a9ccaf4542e3e957edd533ffb85e4f23a
Source Schema Version 1.1
Temporal 2021-07-01/2021-11-01

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