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    • Data and analysis scripts for: Prolonged soybean absence in the field selects for rhizobia that accumulate more polyhydroxybutyrate during symbiosis

    Metadata Updated: December 2, 2025

    During symbiosis, C that rhizobia respire to power N fixation can be stored as polyhydroxybutyrate (PHB), shown to support rhizobia survival under laboratory starvation. We collected soil in 2015 from four replicate plots per treatment in two long-term experiments at Waseca, Minnesota. Treatments differed in the intervals between soybean (Glycine max (L.) Merr.) hosts. We measured PHB accumulation in eight nodules per plant from four soybean (cv. ‘MN0095’) trap plants per soil sample. Trap plants were arranged in a greenhouse, common-garden experiment, and PHB accumulation was measured using flow cytometry. Treatments sampled after long intervals without soybean (greater than two years) showed a greater relative abundance of high-PHB strains. Treatments sampled after the first year of soybean following five years of a non-host crop showed a decreased relative abundance of high-PHB strains, compared to treatments sampled after long intervals without soybean. The latter result is consistent with the hypothesis (not tested directly here) that some high-PHB strains were “sanctioned” by plants as less-beneficial. Our results suggest that rhizobia strains with the tendency to allocate more C to N fixation at the expense of PHB accumulation may be less likely to persist where soybean is grown infrequently or where soil conditions make PHB particularly valuable. However, with typical two-year rotations in Minnesota, differences in PHB storage are unlikely to have a major effect on the relative survival of strains.See README.md for a detailed description of data files and scripts.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: Creative Commons Attribution

    Downloads & Resources

    Dates

    Metadata Created Date May 31, 2024
    Metadata Updated Date December 2, 2025
    Data Update Frequency irregular

    Metadata Source

    Harvested from USDA JSON

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date May 31, 2024
    Metadata Updated Date December 2, 2025
    Publisher Agricultural Research Service
    Maintainer
    Identifier 10.15482/USDA.ADC/25521448.v1
    Data Last Modified 2025-11-22
    Public Access Level public
    Data Update Frequency irregular
    Bureau Code 005:18
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id bfc59635-0a31-4256-b04e-175a2725a0cd
    Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
    Harvest Source Title USDA JSON
    License https://creativecommons.org/licenses/by/4.0/
    Old Spatial {"type": "Point", "coordinates": -93.528657, 44.069167}
    Program Code 005:040
    Source Datajson Identifier True
    Source Hash fd52d25cb4200a7f876c5124af74bef40c1ca09de7f38f084b9ead4164a8df83
    Source Schema Version 1.1
    Spatial {"type": "Point", "coordinates": -93.528657, 44.069167}
    Temporal 1982-05-01/2016-05-01

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