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CottonGen Primer3
Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:
oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,
PCR product size,
positional constraints within the source (template) sequence, and
possibilities for ectopic priming (amplifying the wrong sequence)
many other constraints.
All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.
Resources in this dataset:Resource Title: Website Pointer for CottonGen Primer3. File Name: Web Page, url: https://www.cottongen.org/tools/primer3
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "005:18" ] |
| contactPoint |
{ "fn": "Main, Dorrie", "hasEmail": "mailto:dorrie@wsu.edu" } |
| description | <p>Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:</p> <ul> <li> <p>oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,</p> </li> <li> <p>PCR product size,</p> </li> <li> <p>positional constraints within the source (template) sequence, and</p> </li> <li> <p>possibilities for ectopic priming (amplifying the wrong sequence)</p> </li> <li> <p>many other constraints.</p> </li> </ul> <p>All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.</p> <div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Website Pointer for CottonGen Primer3.</p> <p>File Name: Web Page, url: <a href="https://www.cottongen.org/tools/primer3" target="_blank">https://www.cottongen.org/tools/primer3</a> </p></li></ul> |
| distribution |
[ { "@type": "dcat:Distribution", "title": "https://www.cottongen.org/tools/primer3", "mediaType": "text/html", "downloadURL": "https://www.cottongen.org/tools/primer3" } ] |
| identifier | 10113/AA23039 |
| keyword |
[ "ARS", "CottonGen", "NP301", "Online database", "Primer3", "data.gov" ] |
| license | https://www.usa.gov/publicdomain/label/1.0/ |
| modified | 2024-02-13 |
| programCode |
[ "005:040" ] |
| publisher |
{ "name": "Agricultural Research Service", "@type": "org:Organization" } |
| title | CottonGen Primer3 |
