CottonGen Primer3

Metadata Updated: February 4, 2022

Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:

oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,

PCR product size,

positional constraints within the source (template) sequence, and

possibilities for ectopic priming (amplifying the wrong sequence)

many other constraints.

All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.

Access & Use Information

Public: This dataset is intended for public access and use. License: Creative Commons CCZero

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Dates

Metadata Created Date	November 10, 2020
Metadata Updated Date	February 4, 2022

Metadata Source

Data.json Data.json Metadata
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Harvested from USDA JSON

Additional Metadata

Resource Type	Dataset
Metadata Created Date	November 10, 2020
Metadata Updated Date	February 4, 2022
Publisher	Agricultural Research Service
Maintainer	Main, Dorrie
Identifier	4a131184-c0cb-4ec0-8429-b6e26c5596b9
Data Last Modified	2022-01-31
Public Access Level	public
Bureau Code	005:18
Metadata Context	https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Schema Version	https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby	https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id	0ead0a44-312f-4aa0-b247-ebbecc7a0605
Harvest Source Id	d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
Harvest Source Title	USDA JSON
License	https://creativecommons.org/publicdomain/zero/1.0/
Program Code	005:040
Source Datajson Identifier	True
Source Hash	50bead97c6ef3ac21873cf82444670ab62e08dae
Source Schema Version	1.1

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