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CottonGen Primer3

Metadata Updated: February 4, 2022

Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:

oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,

PCR product size,

positional constraints within the source (template) sequence, and

possibilities for ectopic priming (amplifying the wrong sequence)

many other constraints.

All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.

Access & Use Information

Public: This dataset is intended for public access and use. License: Creative Commons CCZero

Downloads & Resources


Metadata Created Date November 10, 2020
Metadata Updated Date February 4, 2022

Metadata Source

Harvested from USDA JSON

Additional Metadata

Resource Type Dataset
Metadata Created Date November 10, 2020
Metadata Updated Date February 4, 2022
Publisher Agricultural Research Service
Identifier 4a131184-c0cb-4ec0-8429-b6e26c5596b9
Data Last Modified 2022-01-31
Public Access Level public
Bureau Code 005:18
Metadata Context
Schema Version
Catalog Describedby
Harvest Object Id 0ead0a44-312f-4aa0-b247-ebbecc7a0605
Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
Harvest Source Title USDA JSON
Program Code 005:040
Source Datajson Identifier True
Source Hash 50bead97c6ef3ac21873cf82444670ab62e08dae
Source Schema Version 1.1

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