These sets of automated serial sectioning electron backscatter diffraction (EBSD) and X-ray computed tomography (XRCT) measurements were designed to produce spatially registered, multi-modal datasets within a region of interest (ROI) of an additive manufacturing benchmark test series (AM Bench) nickel alloy 718 specimen produced using laser powder bed fusion (PBF-LB) additive manufacturing (AM). The serial sectioning data are in the form of 2D slices which were reconstructed and registered to make 3D data sets that can be analyzed and visualized using open source software. The ROI includes part of a 2.5 mm leg and extends into the baseplate. These measurements are part of an extended dataset associated with the AMB2022-01 set of AM Bench measurements. AMB2022-01 includes powder characterization, detailed information about the build geometry and process, in situ measurements during the build, ex situ measurements of the residual stresses, part distortion following partial removal from the build plate, location-specific microstructure characterization from as-built and heat treated specimens, and microstructure evolution during post-build heat treatments. Detailed descriptions of the AMB2022-01 benchmark measurements and measurement results may be found through the AM Bench webpage at www.nist.gov/ambench. The serial sectioning EBSD and XRCT data in this data publication are also spatially registered with the detailed build file and in situ thermography measurements obtained during the build.