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A Sensitivity Analysis of Methodological Variables Associated with Microbiome Measurements

Published by National Institute of Standards and Technology | National Institute of Standards and Technology | Catalog Last Checked: August 03, 2026 at 03:52 AM | Dataset Last Updated: October 05, 2023
This repository provides the raw data, analysis code, and results generated during a systematic evaluation of the impact of selected experimental protocol choices on the metagenomic sequencing analysis of microbiome samples. Briefly, a full factorial experimental design was implemented varying biological sample (n=5), operator (n=2), lot (n=2), extraction kit (n=2), 16S variable region (n=2), and reference database (n=3), and the main effects were calculated and compared between parameters (bias effects) and samples (real biological differences). A full description of the effort is provided in the associated publication.

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Complete Metadata
@type dcat:Dataset
accessLevel public
bureauCode 
[
  "006:55"
]
contactPoint 
{
  "fn": "Samuel P. Forry",
  "hasEmail": "mailto:samuel.forry@nist.gov"
}
description This repository provides the raw data, analysis code, and results generated during a systematic evaluation of the impact of selected experimental protocol choices on the metagenomic sequencing analysis of microbiome samples. Briefly, a full factorial experimental design was implemented varying biological sample (n=5), operator (n=2), lot (n=2), extraction kit (n=2), 16S variable region (n=2), and reference database (n=3), and the main effects were calculated and compared between parameters (bias effects) and samples (real biological differences). A full description of the effort is provided in the associated publication.
distribution 
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  },
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]
identifier ark:/88434/mds2-3092
issued 2023-11-16
keyword 
[
  "design of experiments",
  "full factorial",
  "metagenomic sequencing",
  "microbiome"
]
landingPage https://data.nist.gov/od/id/mds2-3092
language 
[
  "en"
]
license https://www.nist.gov/open/license
modified 2023-10-05 00:00:00
programCode 
[
  "006:045"
]
publisher 
{
  "name": "National Institute of Standards and Technology",
  "@type": "org:Organization"
}
theme 
[
  "Bioscience:Microbial measurements"
]
title A Sensitivity Analysis of Methodological Variables Associated with Microbiome Measurements

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