{
  "@type": "dcat:Dataset",
  "accessLevel": "public",
  "bureauCode": [
    "006:55"
  ],
  "contactPoint": {
    "fn": "Samuel P. Forry",
    "hasEmail": "mailto:samuel.forry@nist.gov"
  },
  "description": "This repository provides the raw data, analysis code, and results generated during a systematic evaluation of the impact of selected experimental protocol choices on the metagenomic sequencing analysis of microbiome samples. Briefly, a full factorial experimental design was implemented varying biological sample (n=5), operator (n=2), lot (n=2), extraction kit (n=2), 16S variable region (n=2), and reference database (n=3), and the main effects were calculated and compared between parameters (bias effects) and samples (real biological differences). A full description of the effort is provided in the associated publication.",
  "distribution": [
    {
      "description": "R code for analysis and figure generation.",
      "downloadURL": "https://data.nist.gov/od/ds/mds2-3092/resources.zip",
      "format": "R markdown document.",
      "mediaType": "application/zip",
      "title": "resources"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-3092/00README.rtf",
      "mediaType": "application/rtf",
      "title": "00README"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-3092/DataAnalysis.Rmd",
      "mediaType": "application/octet-stream",
      "title": "DataAnalysis"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-3092/ProcessingRawReads.Rmd",
      "mediaType": "application/octet-stream",
      "title": "ProcessingRawReads"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-3092/dada2_output%2Fv3_taxa_tab3Silvav132.txt",
      "mediaType": "text/plain",
      "title": "dada2_output/v3_taxa_tab3Silvav132"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-3092/dada2_output%2Fv3_taxa_tab3Silvav138.txt",
      "mediaType": "text/plain",
      "title": "dada2_output/v3_taxa_tab3Silvav138"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-3092/dada2_output%2Fv3_taxa_tab3rdp18.txt",
      "mediaType": "text/plain",
      "title": "dada2_output/v3_taxa_tab3rdp18"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-3092/dada2_output%2Fv4_taxa_tab3Silvav132.txt",
      "mediaType": "text/plain",
      "title": "dada2_output/v4_taxa_tab3Silvav132"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-3092/dada2_output%2Fv4_taxa_tab3Silvav138.txt",
      "mediaType": "text/plain",
      "title": "dada2_output/v4_taxa_tab3Silvav138"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-3092/dada2_output%2Fv4_taxa_tab3rdp18.txt",
      "mediaType": "text/plain",
      "title": "dada2_output/v4_taxa_tab3rdp18"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-3092/raw_reads.zip",
      "mediaType": "application/zip",
      "title": "raw_reads"
    }
  ],
  "identifier": "ark:/88434/mds2-3092",
  "issued": "2023-11-16",
  "keyword": [
    "design of experiments",
    "full factorial",
    "metagenomic sequencing",
    "microbiome"
  ],
  "landingPage": "https://data.nist.gov/od/id/mds2-3092",
  "language": [
    "en"
  ],
  "license": "https://www.nist.gov/open/license",
  "modified": "2023-10-05 00:00:00",
  "programCode": [
    "006:045"
  ],
  "publisher": {
    "@type": "org:Organization",
    "name": "National Institute of Standards and Technology"
  },
  "theme": [
    "Bioscience:Microbial measurements"
  ],
  "title": "A Sensitivity Analysis of Methodological Variables Associated with Microbiome Measurements"
}