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Federal
Subunit modification and association in VR1 ion channels
U.S. Department of Health & Human Services —
Background The capsaicin (vanilloid) receptor, VR1, is an agonist-activated ion channel expressed by sensory neurons that serves as a detector of chemical and thermal... -
Federal
Retroviral vectors for establishing tetracycline-regulated gene expression in an otherwise recalcitrant cell line
U.S. Department of Health & Human Services —
Background Tetracycline-regulated systems have been used to control the expression of heterologous genes in such diverse organisms as yeast, plants, flies and mice.... -
Federal
Sequence requirements for RNA strand transfer during nidovirus discontinuous subgenomic RNA synthesis
U.S. Department of Health & Human Services —
Nidovirus subgenomic mRNAs contain a leader sequence derived from the 5′ end of the genome fused to different sequences (‘bodies’) derived from the 3′ end. Their... -
Federal
Blood borne transit of CJD from brain to gut at early stages of infection
U.S. Department of Health & Human Services —
Background In Creutzfeldt-Jakob disease (CJD) and other related transmissible spongiform encephalopathies it is critical to understand the various pathways by which... -
Federal
Rational use of computerized protocols in the intensive care unit
U.S. Department of Health & Human Services —
Excess information in complex ICU environments exceeds human decision making limits, increasing the likelihood of clinical errors. Explicit decision-support tools... -
Federal
Expression of p53, inducible nitric oxide synthase and vascular endothelial growth factor in gastric precancerous and cancerous lesions: correlation with clinical features
U.S. Department of Health & Human Services —
Background The growth and metastasis of tumors depend on the development of an adequate blood supply via angiogenesis. Recent studies indicate that the inducible... -
Federal
Treatment of achalasia: the short-term response to botulinum toxin injection seems to be independent of any kind of pretreatment
U.S. Department of Health & Human Services —
Background It has been suggested that intrasphincteric injection of botulinum toxin (BTX) may represent an alternative therapy to balloon dilatation in achalasia. The... -
Federal
Detection of mutations in the dystrophin gene via automated DHPLC screening and direct sequencing
U.S. Department of Health & Human Services —
Background Currently molecular diagnostic laboratories focus only on the identification of large deletion and duplication mutations (spanning one exon or more) for... -
Federal
Acquisition of tolerance against oxidative damage in
U.S. Department of Health & Human Services —
Background Living cells constantly sense and adapt to redox shifts by the induction of genes whose products act to maintain the cellular redox environment. In the... -
Federal
The Metal Coordination of sCD39 during ATP Hydrolysis
U.S. Department of Health & Human Services —
Background The hydrolysis of ATP and ADP by ecto-nucleoside triphosphate diphosphohydrolase 1 (CD39) requires divalent cations, like Ca2+ and Mg2+. In spite of... -
Federal
Agitation in the ICU: part one Anatomical and physiologic basis for the agitated state
U.S. Department of Health & Human Services —
Agitation in the ICU: part one Anatomical and physiologic basis for the agitated state -
Federal
Multi-exon deletions of the
U.S. Department of Health & Human Services —
Background Mutations in the fibrillin -1 gene (FBN1) cause Marfan syndrome (MFS), an autosomal dominant multi-system connective tissue disorder. The 200 different... -
Federal
Upregulation of the SERCA-type Ca
U.S. Department of Health & Human Services —
Background Ca2+-ATPases of endoplasmic reticulum (SERCAs) are responsible for maintenance of the micro- to millimolar Ca2+ ion concentrations within the endoplasmic... -
Federal
Flow cytometry analysis of the effect of allopurinol and the dinitroaniline compound (Chloralin) on the viability and proliferation of
U.S. Department of Health & Human Services —
Background Leishmaniasis is a major parasitic disease in the tropical regions. However, Leishmania infantum has recently emerged as a very important cause of... -
Federal
Regulation of prostaglandin synthesis and cell adhesion by a tryptophan catabolizing enzyme
U.S. Department of Health & Human Services —
Background The tryptophan catabolizing enzyme, indoleamine 2,3, dioxygenase (IDO) is one of two mammalian enzymes, which can catabolize the rarest essential amino... -
Federal
Combinatorial diversity of fission yeast SCF ubiquitin ligases by homo- and heterooligomeric assemblies of the F-box proteins Pop1p and Pop2p
U.S. Department of Health & Human Services —
Background SCF ubiquitin ligases share the core subunits cullin 1, SKP1, and HRT1/RBX1/ROC1, which associate with different F-box proteins. F-box proteins bind... -
Federal
Leukocyte adhesion and recruitment, and alpha-1-antitrypsin deficiency: a report from ATS 2001, May 18-23, San Francisco
U.S. Department of Health & Human Services —
The program at this year's American Thoracic Society international conference included over 300 scientific and clinical symposia. In this report I have reviewed the... -
Federal
Delineation of RAID1, the RACK1 interaction domain located within the unique N-terminal region of the cAMP-specific phosphodiesterase, PDE4D5
U.S. Department of Health & Human Services —
Background The cyclic AMP specific phosphodiesterase, PDE4D5 interacts with the β-propeller protein RACK1 to form a signaling scaffold complex in cells. Two-hybrid... -
Federal
Cellophane based mini-prep method for DNA extraction from the filamentous fungus
U.S. Department of Health & Human Services —
Background Methods for the extraction of DNA from filamentous fungi are frequently laborious and time consuming because most of the available protocols include... -
Federal
Cellular responses to
U.S. Department of Health & Human Services —
Background Plasmodium falciparum erythrocyte membrane protein-1, a variant antigen of the malaria parasite, is potentially a target for the immune response. It would...