Zebrafish larvae submitted to simulated microgravity on a clinostat (CLINO) at 5dpf for one day
The general objective of the study was to determine modulation of gene expression by environmental factors specifically simulation of microgravity with a special emphasis on bone formation. For this reason the specific period of treatment was chosen between 5-6 days post-fertilization (dpf) when bone formation and calcification are taking place. Zebrafish larvae were placed at 5 dpf into a clinostat (CLINO) for 24 hours which was shown to simulate microgravity by the specific rotational movements it generates. We show that CLINO exposure causes a clear decrease of bone formation as illustrated by cranial skeleton staining of the bone matrix by Alizarin Red by morphometric analysis of the resulting images. Thus a whole genome micro-array experiment was conducted to identify genes that may be involved in the observed effect on bone formation.
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Zebrafish larvae submitted to simulated microgravity on a clinostat (CLINO) at 5dpf for one day
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[ "026:00" ] |
| contactPoint |
{ "fn": "GeneLab Outreach", "@type": "vcard:Contact", "hasEmail": "mailto:genelab-outreach@lists.nasa.gov" } |
| description | The general objective of the study was to determine modulation of gene expression by environmental factors specifically simulation of microgravity with a special emphasis on bone formation. For this reason the specific period of treatment was chosen between 5-6 days post-fertilization (dpf) when bone formation and calcification are taking place. Zebrafish larvae were placed at 5 dpf into a clinostat (CLINO) for 24 hours which was shown to simulate microgravity by the specific rotational movements it generates. We show that CLINO exposure causes a clear decrease of bone formation as illustrated by cranial skeleton staining of the bone matrix by Alizarin Red by morphometric analysis of the resulting images. Thus a whole genome micro-array experiment was conducted to identify genes that may be involved in the observed effect on bone formation. |
| distribution |
[ { "@type": "dcat:Distribution", "title": "Zebrafish larvae submitted to simulated microgravity on a clinostat (CLINO) at 5dpf for one day", "format": "HTML", "mediaType": "text/html", "description": "GeneLab Study Page", "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-76" } ] |
| identifier | nasa_genelab_GLDS-76_ps96-ni2k |
| issued | 2018-06-26 |
| keyword |
[ "array-scanning-and-feature-extraction", "growth-protocol", "labeling", "normalization-data-transformation", "nucleic-acid-hybridization", "p-mtab-48667", "p-mtab-48668", "p-mtab-48669", "p-mtab-48670", "p-mtab-48671", "p-mtab-48672", "p-mtab-48673", "rna-extraction", "stress", "treatment-protocol" ] |
| landingPage | https://data.nasa.gov/dataset/zebrafish-larvae-submitted-to-simulated-microgravity-on-a-clinostat-clino-at-5dpf-for-one- |
| modified | 2025-04-23 |
| programCode |
[ "026:005" ] |
| publisher |
{ "name": "National Aeronautics and Space Administration", "@type": "org:Organization" } |
| theme |
[ "Earth Science" ] |
| title | Zebrafish larvae submitted to simulated microgravity on a clinostat (CLINO) at 5dpf for one day |
