Transcriptomic analysis of skin from mice subjected to chronic low-dose radiation hindlimb unloading or a combination of both
The purpose of this study was to evaluate transcriptional changes in mouse skin using a ground-based model for spaceflight. This model includes prolonged unloading and low-dose irradiation. Low-dose-rate gamma-radiation was delivered to 6-month old female C57BL/6J mice using 57Co plates (0.04 Gy) to simulate the radiation environment of spaceflight. Anti-orthostatic tail suspension was used to model the unloading fluid shift and physiological stress aspects of the microgravity component of spaceflight. Mice were hindlimb suspended and/or irradiated for 21 days. Mice were euthanized and dorsal skin was collected 7 days following treatment. RNA sequencing data was generated to assess transcriptional changes in these skin samples.
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Transcriptomic analysis of skin from mice subjected to chronic low-dose radiation hindlimb unloading or a combination of both
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[ "026:00" ] |
| contactPoint |
{ "fn": "GeneLab Outreach", "@type": "vcard:Contact", "hasEmail": "mailto:genelab-outreach@lists.nasa.gov" } |
| description | The purpose of this study was to evaluate transcriptional changes in mouse skin using a ground-based model for spaceflight. This model includes prolonged unloading and low-dose irradiation. Low-dose-rate gamma-radiation was delivered to 6-month old female C57BL/6J mice using 57Co plates (0.04 Gy) to simulate the radiation environment of spaceflight. Anti-orthostatic tail suspension was used to model the unloading fluid shift and physiological stress aspects of the microgravity component of spaceflight. Mice were hindlimb suspended and/or irradiated for 21 days. Mice were euthanized and dorsal skin was collected 7 days following treatment. RNA sequencing data was generated to assess transcriptional changes in these skin samples. |
| distribution |
[ { "@type": "dcat:Distribution", "title": "Transcriptomic analysis of skin from mice subjected to chronic low-dose radiation hindlimb unloading or a combination of both", "format": "HTML", "mediaType": "text/html", "description": "GeneLab Study Page", "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-237" } ] |
| identifier | nasa_genelab_GLDS-237_brjx-sswq |
| issued | 2021-05-21 |
| keyword |
[ "data-transformation", "genelab-rnaseq-data-processing-protocol", "hindlimb-unloading", "ionizing-radiation", "library-construction", "nucleic-acid-extraction", "nucleic-acid-sequencing", "sample-collection", "spike-in-protocol", "treatment-protocol" ] |
| landingPage | https://data.nasa.gov/dataset/transcriptomic-analysis-of-skin-from-mice-subjected-to-chronic-low-dose-radiation-hindlimb |
| license | http://www.usa.gov/publicdomain/label/1.0/ |
| modified | 2025-04-23 |
| programCode |
[ "026:005" ] |
| publisher |
{ "name": "National Aeronautics and Space Administration", "@type": "org:Organization" } |
| theme |
[ "Earth Science" ] |
| title | Transcriptomic analysis of skin from mice subjected to chronic low-dose radiation hindlimb unloading or a combination of both |
