RNA-seq and ATAC-seq data from porcine innate immune cells associated with BCG-induced trained immunity
This dataset contains RNA-seq and ATAC-seq data generated from CD14+ monocytes isolated from neonatal piglets inoculated with BCG (Bacillus Calmette-Guérin) or PBS control. Blood was collected at 3 and 6 weeks post-inoculation. Monocytes were stimulated ex vivo with LPS, Pam3CSK4, or media alone. RNA-seq was performed using the Lexogen QuantSeq 3' mRNA-seq protocol on a NovaSeq 6000. ATAC-seq was performed on monocytes from the 6-week timepoint to assess chromatin accessibility. Data support investigation of epigenetic and transcriptional mechanisms of BCG-induced trained immunity in pigs. Raw sequencing data are deposited at NCBI SRA under BioProject PRJNA1472623.
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accessRights | public |
| accrualPeriodicity | irregular |
| bureauCode |
[ "005:18", "005:20" ] |
| contactPoint |
{ "fn": "Loving, Crystal", "hasEmail": "mailto:crystal.loving@usda.gov" } |
| description | <p dir="ltr">This dataset contains RNA-seq and ATAC-seq data generated from CD14+ monocytes isolated from neonatal piglets inoculated with BCG (Bacillus Calmette-Guérin) or PBS control. Blood was collected at 3 and 6 weeks post-inoculation. Monocytes were stimulated ex vivo with LPS, Pam3CSK4, or media alone. RNA-seq was performed using the Lexogen QuantSeq 3' mRNA-seq protocol on a NovaSeq 6000. ATAC-seq was performed on monocytes from the 6-week timepoint to assess chromatin accessibility. Data support investigation of epigenetic and transcriptional mechanisms of BCG-induced trained immunity in pigs. Raw sequencing data are deposited at NCBI SRA under BioProject PRJNA1472623.</p> |
| distribution |
[ { "@type": "dcat:Distribution", "title": "https://www.ncbi.nlm.nih.gov/sra/PRJNA1472623", "license": "https://creativecommons.org/publicdomain/zero/1.0/", "mediaType": "text/html", "downloadURL": "https://www.ncbi.nlm.nih.gov/sra/PRJNA1472623" } ] |
| identifier | 10779/USDA.ADC.32670663.v1 |
| keyword |
[ "BCG", "chromatin accessibility", "epigenetics", "immune cells", "pig", "protein", "trained immunity", "transcriptome" ] |
| license | https://creativecommons.org/publicdomain/zero/1.0/ |
| modified | 2026-08-07 |
| programCode |
[ "005:040" ] |
| publisher |
{ "name": "Agricultural Research Service", "@type": "org:Organization" } |
| temporal |
[ { "@type": "PeriodOfTime", "endDate": "2021-09-01", "startDate": "2021-07-01" } ] |
| title | RNA-seq and ATAC-seq data from porcine innate immune cells associated with BCG-induced trained immunity |
