New record of California red-legged frog (Rana draytonii) in Whitewater Canyon, Riverside County, CA, USA
In February 2017, a population of California red-legged frog Rana draytonii was discovered in the southern foothills of the San Bernardino Mountains of Riverside County, California, near the edge of the species’ historic distribution. A few days after the first sightings of R. draytonii at the Whitewater Preserve, we conducted a visual daytime search for frogs, tadpoles and egg masses followed by a nighttime eye shine search for adult frogs. The captured frog weighed, measured (snout-to-urostyle [SU]), sampled for tissue and georeferenced using a GPS unit. Survey work was authorized by a U.S. Fish and Wildlife Service Recovery Permit and a California Department of Fish and Wildlife Scientific Collecting Permit.
To better understand the origin of these frogs, we extracted DNA from a small toe clip using a Qiagen® DNeasy Kit (Qiagen Inc., Valencia CA) and Sanger-sequenced a region of the cytochrome b mitochondrial gene (986 base-pairs) that has been used to good effect for identifying regionally specific 67 mtDNA clades (Richmond et al. 2014). Sequencing was performed on a 3730xl DNA Analyzer at Genewiz (La Jolla, CA). We used DnaSP 5.10 (Librado & Rozas 2009) to match the haplotype sequence against a large cytochrome B database representing the full range of R. draytonii.
These data are associated with the following publciation: Backlin, A., Richmond, J., Gallegos, E., Christensen, C., and Fisher, R. (2017). An extirpated lineage of a threatened frog species resurfaces in southern California. Oryx, 1-5. doi:10.1017/S0030605317001168
These data are associated with the following GenBank accession number: MG022156
References:
Richmond, J.Q., Backlin, A.R., Tatarian, P.J., Solvesky, B.G. & Fisher, R.N. (2014) Population declines lead to replicate patterns of internal range structure at the tips of the distribution of the California red-legged frog (Rana draytonii). Biological Conservation, 172, 128-137.
Librado, P. & Rozas, J. (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25, 1451-1452.
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "010:12" ] |
| contactPoint |
{ "fn": "Adam R Backlin", "@type": "vcard:Contact", "hasEmail": "mailto:abacklin@usgs.gov" } |
| description | In February 2017, a population of California red-legged frog Rana draytonii was discovered in the southern foothills of the San Bernardino Mountains of Riverside County, California, near the edge of the species’ historic distribution. A few days after the first sightings of R. draytonii at the Whitewater Preserve, we conducted a visual daytime search for frogs, tadpoles and egg masses followed by a nighttime eye shine search for adult frogs. The captured frog weighed, measured (snout-to-urostyle [SU]), sampled for tissue and georeferenced using a GPS unit. Survey work was authorized by a U.S. Fish and Wildlife Service Recovery Permit and a California Department of Fish and Wildlife Scientific Collecting Permit. To better understand the origin of these frogs, we extracted DNA from a small toe clip using a Qiagen® DNeasy Kit (Qiagen Inc., Valencia CA) and Sanger-sequenced a region of the cytochrome b mitochondrial gene (986 base-pairs) that has been used to good effect for identifying regionally specific 67 mtDNA clades (Richmond et al. 2014). Sequencing was performed on a 3730xl DNA Analyzer at Genewiz (La Jolla, CA). We used DnaSP 5.10 (Librado & Rozas 2009) to match the haplotype sequence against a large cytochrome B database representing the full range of R. draytonii. These data are associated with the following publciation: Backlin, A., Richmond, J., Gallegos, E., Christensen, C., and Fisher, R. (2017). An extirpated lineage of a threatened frog species resurfaces in southern California. Oryx, 1-5. doi:10.1017/S0030605317001168 These data are associated with the following GenBank accession number: MG022156 References: Richmond, J.Q., Backlin, A.R., Tatarian, P.J., Solvesky, B.G. & Fisher, R.N. (2014) Population declines lead to replicate patterns of internal range structure at the tips of the distribution of the California red-legged frog (Rana draytonii). Biological Conservation, 172, 128-137. Librado, P. & Rozas, J. (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25, 1451-1452. |
| distribution |
[ { "@type": "dcat:Distribution", "title": "Digital Data", "format": "XML", "accessURL": "https://doi.org/10.5066/F7C82870", "mediaType": "application/http", "description": "Landing page for access to the data" }, { "@type": "dcat:Distribution", "title": "Original Metadata", "format": "XML", "mediaType": "text/xml", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.59a70105e4b0fd9b77cf6b75.xml" } ] |
| identifier | http://datainventory.doi.gov/id/dataset/USGS_59a70105e4b0fd9b77cf6b75 |
| keyword |
[ "Amphibian conservation", "Frog", "Rana draytonii", "USGS:59a70105e4b0fd9b77cf6b75", "amphibians", "biota" ] |
| modified | 2020-08-27T00:00:00Z |
| publisher |
{ "name": "U.S. Geological Survey", "@type": "org:Organization" } |
| spatial | -116.96362495422365, 34.024351833551606, -116.93663120269777, 34.046116798378975 |
| theme |
[ "geospatial" ] |
| title | New record of California red-legged frog (Rana draytonii) in Whitewater Canyon, Riverside County, CA, USA |
