{"accessLevel": "public", "bureauCode": ["010:12"], "contactPoint": {"@type": "vcard:Contact", "fn": "Adam R Backlin", "hasEmail": "mailto:abacklin@usgs.gov"}, "description": "In February 2017, a population of California red-legged frog Rana draytonii was discovered in the southern foothills of the San Bernardino Mountains of Riverside County, California, near the edge of the species\u2019 historic distribution. A few days after the first sightings of R. draytonii at the Whitewater Preserve, we conducted a visual daytime search for frogs, tadpoles and egg masses followed by a nighttime eye shine search for adult frogs. The captured frog weighed, measured (snout-to-urostyle [SU]), sampled for tissue and georeferenced using a GPS unit. Survey work was authorized by a U.S. Fish and Wildlife Service Recovery Permit and a California Department of Fish and Wildlife Scientific Collecting Permit.\nTo better understand the origin of these frogs, we extracted DNA from a small toe clip using a Qiagen\u00ae DNeasy Kit (Qiagen Inc., Valencia CA) and Sanger-sequenced a region of the cytochrome b mitochondrial gene (986 base-pairs) that has been used to good effect for identifying regionally specific 67 mtDNA clades (Richmond et al. 2014). Sequencing was performed on a 3730xl DNA Analyzer at Genewiz (La Jolla, CA). We used DnaSP 5.10 (Librado & Rozas 2009) to match the haplotype sequence against a large cytochrome B database representing the full range of R. draytonii. \nThese data are associated with the following publciation: Backlin, A., Richmond, J., Gallegos, E., Christensen, C., and Fisher, R. (2017). An extirpated lineage of a threatened frog species resurfaces in southern California. Oryx, 1-5. doi:10.1017/S0030605317001168\nThese data are associated with the following GenBank accession number: MG022156 \nReferences:\nRichmond, J.Q., Backlin, A.R., Tatarian, P.J., Solvesky, B.G. & Fisher, R.N. (2014) Population declines lead to replicate patterns of internal range structure at the tips of the distribution of the California red-legged frog (Rana draytonii). Biological Conservation, 172, 128-137.\nLibrado, P. & Rozas, J. (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25, 1451-1452.", "distribution": [{"@type": "dcat:Distribution", "accessURL": "https://doi.org/10.5066/F7C82870", "description": "Landing page for access to the data", "format": "XML", "mediaType": "application/http", "title": "Digital Data"}, {"@type": "dcat:Distribution", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.59a70105e4b0fd9b77cf6b75.xml", "format": "XML", "mediaType": "text/xml", "title": "Original Metadata"}], "identifier": "http://datainventory.doi.gov/id/dataset/USGS_59a70105e4b0fd9b77cf6b75", "keyword": ["Amphibian conservation", "Frog", "Rana draytonii", "USGS:59a70105e4b0fd9b77cf6b75", "amphibians", "biota"], "modified": "2020-08-27T00:00:00Z", "publisher": {"@type": "org:Organization", "name": "U.S. Geological Survey"}, "spatial": "-116.96362495422365, 34.024351833551606, -116.93663120269777, 34.046116798378975", "theme": ["geospatial"], "title": "New record of California red-legged frog (Rana draytonii) in Whitewater Canyon, Riverside County, CA, USA"}