Multi-omic investigations of mouse liver subjected to simulated spaceflight freezing and storage protocols
This study compares standard laboratory protocols for tissue freezing and storage with a simulation of the delayed processing of liver specimens and long-term storage protocols used during the Rodent Research-1 (RR-1) payload. Liver samples from twelve-week old female C57/BL6 mice (snap frozen vs. 25 min. slow-frozen and stored for 3 days vs. 1 year) received from the RR-1 project were processed for extraction of RNA DNA and protein. RNA-seq whole genome bisulfite sequencing and mass spectrometry proteomics were performed. These multi-omics datasets will help distinguish the effects of freezing and storage time that could confound the interpretation of the Rodent Research-1 datasets.
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Multi-omic investigations of mouse liver subjected to simulated spaceflight freezing and storage protocols
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[ "026:00" ] |
| contactPoint |
{ "fn": "GeneLab Outreach", "@type": "vcard:Contact", "hasEmail": "mailto:genelab-outreach@lists.nasa.gov" } |
| description | This study compares standard laboratory protocols for tissue freezing and storage with a simulation of the delayed processing of liver specimens and long-term storage protocols used during the Rodent Research-1 (RR-1) payload. Liver samples from twelve-week old female C57/BL6 mice (snap frozen vs. 25 min. slow-frozen and stored for 3 days vs. 1 year) received from the RR-1 project were processed for extraction of RNA DNA and protein. RNA-seq whole genome bisulfite sequencing and mass spectrometry proteomics were performed. These multi-omics datasets will help distinguish the effects of freezing and storage time that could confound the interpretation of the Rodent Research-1 datasets. |
| distribution |
[ { "@type": "dcat:Distribution", "title": "Multi-omic investigations of mouse liver subjected to simulated spaceflight freezing and storage protocols", "format": "HTML", "mediaType": "text/html", "description": "GeneLab Study Page", "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-49" } ] |
| identifier | nasa_genelab_GLDS-49_56j4-37dc |
| issued | 2018-06-26 |
| keyword |
[ "animal-and-tissue-sampling", "extraction", "freezing-profile", "labeling", "library-construction", "mass-spectrometry", "nucleic-acid-extraction", "nucleic-acid-sequencing", "protein-extraction", "proteomic-labeling", "sample-collection", "tissue-storage-time" ] |
| landingPage | https://data.nasa.gov/dataset/multi-omic-investigations-of-mouse-liver-subjected-to-simulated-spaceflight-freezing-and-s |
| modified | 2025-04-23 |
| programCode |
[ "026:005" ] |
| publisher |
{ "name": "National Aeronautics and Space Administration", "@type": "org:Organization" } |
| theme |
[ "Earth Science" ] |
| title | Multi-omic investigations of mouse liver subjected to simulated spaceflight freezing and storage protocols |
