{"@type": "dcat:Dataset", "accessLevel": "public", "accrualPeriodicity": "irregular", "bureauCode": ["026:00"], "contactPoint": {"@type": "vcard:Contact", "fn": "GeneLab Outreach", "hasEmail": "mailto:genelab-outreach@lists.nasa.gov"}, "description": "This study compares standard laboratory protocols for tissue freezing and storage with a simulation of the delayed processing of liver specimens and long-term storage protocols used during the Rodent Research-1 (RR-1) payload. Liver samples from twelve-week old female C57/BL6 mice (snap frozen vs. 25 min. slow-frozen and stored for 3 days vs. 1 year) received from the RR-1 project were processed for extraction of RNA DNA and protein. RNA-seq whole genome bisulfite sequencing and mass spectrometry proteomics were performed. These multi-omics datasets will help distinguish the effects of freezing and storage time that could confound the interpretation of the Rodent Research-1 datasets.", "distribution": [{"@type": "dcat:Distribution", "description": "GeneLab Study Page", "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-49", "format": "HTML", "mediaType": "text/html", "title": "Multi-omic investigations of mouse liver subjected to simulated spaceflight freezing and storage protocols"}], "identifier": "nasa_genelab_GLDS-49_56j4-37dc", "issued": "2018-06-26", "keyword": ["animal-and-tissue-sampling", "extraction", "freezing-profile", "labeling", "library-construction", "mass-spectrometry", "nucleic-acid-extraction", "nucleic-acid-sequencing", "protein-extraction", "proteomic-labeling", "sample-collection", "tissue-storage-time"], "landingPage": "https://data.nasa.gov/dataset/multi-omic-investigations-of-mouse-liver-subjected-to-simulated-spaceflight-freezing-and-s", "modified": "2025-04-23", "programCode": ["026:005"], "publisher": {"@type": "org:Organization", "name": "National Aeronautics and Space Administration"}, "theme": ["Earth Science"], "title": "Multi-omic investigations of mouse liver subjected to simulated spaceflight freezing and storage protocols"}