{
  "@type": "dcat:Dataset",
  "accessLevel": "public",
  "accrualPeriodicity": "irregular",
  "bureauCode": [
    "026:00"
  ],
  "contactPoint": {
    "@type": "vcard:Contact",
    "fn": "GeneLab Outreach",
    "hasEmail": "mailto:genelab-outreach@lists.nasa.gov"
  },
  "description": "This study compares standard laboratory protocols for tissue freezing and storage with a simulation of the delayed processing of liver specimens and long-term storage protocols used during the Rodent Research-1 (RR-1) payload. Liver samples from twelve-week old female C57/BL6 mice (snap frozen vs. 25 min. slow-frozen and stored for 3 days vs. 1 year) received from the RR-1 project were processed for extraction of RNA DNA and protein. RNA-seq whole genome bisulfite sequencing and mass spectrometry proteomics were performed. These multi-omics datasets will help distinguish the effects of freezing and storage time that could confound the interpretation of the Rodent Research-1 datasets.",
  "distribution": [
    {
      "@type": "dcat:Distribution",
      "description": "GeneLab Study Page",
      "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-49",
      "format": "HTML",
      "mediaType": "text/html",
      "title": "Multi-omic investigations of mouse liver subjected to simulated spaceflight freezing and storage protocols"
    }
  ],
  "identifier": "nasa_genelab_GLDS-49_56j4-37dc",
  "issued": "2018-06-26",
  "keyword": [
    "animal-and-tissue-sampling",
    "extraction",
    "freezing-profile",
    "labeling",
    "library-construction",
    "mass-spectrometry",
    "nucleic-acid-extraction",
    "nucleic-acid-sequencing",
    "protein-extraction",
    "proteomic-labeling",
    "sample-collection",
    "tissue-storage-time"
  ],
  "landingPage": "https://data.nasa.gov/dataset/multi-omic-investigations-of-mouse-liver-subjected-to-simulated-spaceflight-freezing-and-s",
  "modified": "2025-04-23",
  "programCode": [
    "026:005"
  ],
  "publisher": {
    "@type": "org:Organization",
    "name": "National Aeronautics and Space Administration"
  },
  "theme": [
    "Earth Science"
  ],
  "title": "Multi-omic investigations of mouse liver subjected to simulated spaceflight freezing and storage protocols"
}