mRNA expression profile in DLD-1 and MOLT-4 cancer cell lines cultured under Microgravity

Metadata Updated: July 17, 2020

DLD-1 and MOLT-4 cell lines were cultured in a Rotating cell culture system to simulate microgravity and mRNA expression profile was observed in comparison to Static controls. Cells were grown in 10mL rotating vessels in an RCCS and in 60mm Petri dishes (test control respectively).Two replicates of test (Microgravity) and control (static) each from DLD-1 and MOLT-4 were analyzed by microarray. Simulated microgravity affected the solid tumor cell line DLD-1 markedly which showed a higher percentage of dysregulated genes compared to the hematological tumor cell line MOLT-4. Microgravity affects the cell cycle of DLD-1 cells and disturbs expression of cell cycle regulatory gene networks. Multiple microRNA host genes were dysregulated and significantly mir-22 tumor suppressor microRNA is highly upregulated in DLD-1.

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Dates

Metadata Created Date August 1, 2018
Metadata Updated Date July 17, 2020
Data Update Frequency irregular

Metadata Source

Harvested from NASA Data.json

Additional Metadata

Resource Type Dataset
Metadata Created Date August 1, 2018
Metadata Updated Date July 17, 2020
Publisher National Aeronautics and Space Administration
Unique Identifier nasa_genelab_GLDS-125
Maintainer
GeneLab Outreach
Maintainer Email
Public Access Level public
Data Update Frequency irregular
Bureau Code 026:00
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://data.nasa.gov/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id 8e9c8a9f-79b2-4619-b735-2becf4076e8e
Harvest Source Id 39e4ad2a-47ca-4507-8258-852babd0fd99
Harvest Source Title NASA Data.json
Data First Published 2018-06-26
Homepage URL https://data.nasa.gov/d/fway-due9
Data Last Modified 2020-01-29
Program Code 026:005
Source Datajson Identifier True
Source Hash aa0c0a8bc62a0c85a65fbb5e41d6b87f68353089
Source Schema Version 1.1
Category Earth Science

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