In Vitro Metabolism of Imidacloprid and Acetamiprid in Rainbow Trout and Rat - Kolanczyk_A-dv4j_SDMP_20190918

The current study constitutes the first report of the metabolism of imidacloprid and acetamiprid in rainbow trout. Formation of respective metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was validated by analytical methods and demonstrated to be conserved across orders of biological organization in both microsomal and liver slice assays. Michaelis-Menten kinetics were determined for the microsomal conversion of imidacloprid to 5-hydroxy-imidacloprid in rainbow trout (Km=79.2 µM; Vmax=0.75 pmole/min/mg) and rat (Km=158.7 µM; Vmax=38.4 pmole/min/mg). Kinetics for the microsomal demethylation of acetamiprid to N-desmethyl-acetamiprid were determined in the rat (Km=70.9 µM; Vmax=4.10 pmoles/min/mg). N-desmethyl-acetamiprid was found in detectable but below quantifiable levels across the range of test concentrations which precluded a calculation of kinetic rate constants in rainbow trout (RBT). Ultimately, the formation of the metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was conserved across RBT and rat species. This dataset is associated with the following publication: Kolanczyk, R., M. Tapper, B. Sheedy, and J. Serrano. In vitro metabolism of imidacloprid and acetamiprid in rainbow trout and rat. XENOBIOTICA. Taylor & Francis, Inc., Philadelphia, PA, USA, 50(7): 805-814, (2020).

[
  "020:00"
]

{
  "fn": "Richard Kolanczyk",
  "hasEmail": "mailto:kolanczyk.rick@epa.gov"
}

[
  {
    "title": "Imidacloprid acetamiprid trout vs rat data.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1504449/Imidacloprid%20acetamiprid%20trout%20vs%20rat%20data.xlsx"
  }
]

[
  "Metabolism",
  "Rat",
  "acetamiprid",
  "imidacloprid",
  "in vitro",
  "liver slice",
  "microsomes",
  "neonicotinoid",
  "rainbow trout"
]

[
  "020:000"
]

{
  "name": "U.S. EPA Office of Research and Development (ORD)",
  "subOrganizationOf": {
    "name": "U.S. Environmental Protection Agency",
    "subOrganizationOf": {
      "name": "U.S. Government"
    }
  }
}

[
  "https://doi.org/10.1080/00498254.2019.1694197"
]

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