{"accessLevel": "public", "bureauCode": ["020:00"], "contactPoint": {"fn": "Richard Kolanczyk", "hasEmail": "mailto:kolanczyk.rick@epa.gov"}, "description": "The current study constitutes the first report of the metabolism of imidacloprid and acetamiprid in rainbow trout. Formation of respective metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was validated by analytical methods and demonstrated to be conserved across orders of biological organization in both microsomal and liver slice assays. Michaelis-Menten kinetics were determined for the microsomal conversion of imidacloprid to 5-hydroxy-imidacloprid in rainbow trout (Km=79.2 \u00b5M; Vmax=0.75 pmole/min/mg) and rat (Km=158.7 \u00b5M; Vmax=38.4 pmole/min/mg). Kinetics for the microsomal demethylation of acetamiprid to N-desmethyl-acetamiprid were determined in the rat (Km=70.9 \u00b5M; Vmax=4.10 pmoles/min/mg). N-desmethyl-acetamiprid was found in detectable but below quantifiable levels across the range of test concentrations which precluded a calculation of kinetic rate constants in rainbow trout (RBT). Ultimately, the formation of the metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was conserved across  RBT and rat species. \n\nThis dataset is associated with the following publication:\nKolanczyk, R., M. Tapper, B. Sheedy, and J. Serrano. In vitro metabolism of imidacloprid and acetamiprid in rainbow trout and rat.   XENOBIOTICA. Taylor & Francis, Inc., Philadelphia, PA, USA, 50(7): 805-814, (2020).", "distribution": [{"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1504449/Imidacloprid%20acetamiprid%20trout%20vs%20rat%20data.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "Imidacloprid acetamiprid trout vs rat data.xlsx"}], "identifier": "https://doi.org/10.23719/1504449", "keyword": ["Metabolism", "Rat", "acetamiprid", "imidacloprid", "in vitro", "liver slice", "microsomes", "neonicotinoid", "rainbow trout"], "license": "https://pasteur.epa.gov/license/sciencehub-license.html", "modified": "2019-05-15", "programCode": ["020:000"], "publisher": {"name": "U.S. EPA Office of Research and Development (ORD)", "subOrganizationOf": {"name": "U.S. Environmental Protection Agency", "subOrganizationOf": {"name": "U.S. Government"}}}, "references": ["https://doi.org/10.1080/00498254.2019.1694197"], "rights": null, "title": "In Vitro Metabolism of Imidacloprid and Acetamiprid in Rainbow Trout and Rat - Kolanczyk_A-dv4j_SDMP_20190918"}