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Hepatic gene expression transcript counts in liver samples of American kestrels

Metadata Updated: October 28, 2023

A number of brominated flame retardants (BFRs) have been reported to interfere with the thyroid signaling pathway and cause oxidative stress in birds, yet the underlying shifts in gene expression associated with these effects remain poorly understood. In this study, we measured hepatic transcriptional responses of 31 genes in American kestrel hatchlings following in ovo exposure to one of three high-volume alternative BFRs: 1,2-bis(2,4,6-tribromophenoxy) ethane (BTPBE), bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate (TBPH), or 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (EHTBB). Hatchling kestrels exhibited shifts in the expression of genes related to oxidative stress (CYP, GSTA, SOD, GPx), thyroid hormone metabolism and transport (DIO, TTR), lipid and protein metabolism (PPAR, HMGCR, FAB1, LPL), and cytokine-mediated inflammation (TLR, IL-18, IRF7, STAT3, RACK1, CEBPB). Male and female hatchlings differed in which genes were differentially expressed as well as the direction of the effect (up- vs. down-regulation). These results build upon our previous findings of increased oxidative stress and disrupted thyroid signaling pathway in the same hatchlings. Furthermore, our results indicate that inflammatory responses appear to occur in female hatchlings exposed to BTBPE and EHTBB in ovo. Gene expression analysis revealed multiple affected pathways, adding to the growing evidence that sublethal physio-logical effects are complex and are a concern for birds exposed to BTBPE, EHTBB, or TBPH in ovo.

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Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Metadata Created Date August 1, 2023
Metadata Updated Date October 28, 2023

Metadata Source

Harvested from DOI EDI

Additional Metadata

Resource Type Dataset
Metadata Created Date August 1, 2023
Metadata Updated Date October 28, 2023
Publisher U.S. Geological Survey
Identifier USGS:6303ab42d34ed6dc5592a397
Data Last Modified 20230726
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context
Metadata Catalog ID
Schema Version
Catalog Describedby
Harvest Object Id 2f0f0862-ff18-4cda-a428-edc76c4eb471
Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
Harvest Source Title DOI EDI
Metadata Type geospatial
Old Spatial -77.9398,39.0345,-76.7889,39.3582
Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
Source Datajson Identifier True
Source Hash 081b502aa73f921517ecf30aec58a67082d9960d90c640d467d4e22abfc44e3d
Source Schema Version 1.1
Spatial {"type": "Polygon", "coordinates": -77.9398, 39.0345, -77.9398, 39.3582, -76.7889, 39.3582, -76.7889, 39.0345, -77.9398, 39.0345}

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