In the present study, features of gonad metabolite profiles associated with TO in largemouth bass (LMB, Micropterus salmoides) from an impoundment in Georgia (USA) were determined using GC–MS-based metabolomics. Clinical blood biochemical screens were used to evaluate markers of fish health associated with TO. Results suggest that physiological changes in energy expenditure as well as relatively ‘feminized’ gonad lipid and protein metabolism may be related to the occurrence of TO in male LMB, and highlight the need to understand relationships between intersex and physical stressors such as elevated temperature and hypoxia. These results provide novel insight to AOPs associated with TO and identify candidate analytes for biomarker discovery. This dataset is not publicly accessible because: Data belongs to coauthor at University of Georgia. It can be accessed through the following means: Data can be accessed by contacting the corresponding author (bringo@uga.edu). Format: excel.

This dataset is associated with the following publication: Urich, M., W. Henderson, A. MacLeod, L. Yonkos, and R. Bringolf,. Gonad metabolomics and blood biochemical analysis reveal differences associated with testicular oocytes in wild largemouth bass (Micropterus salmoides). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY - PART B: BIOCHEMISTRY & MOLECULAR BIOLOGY. Elsevier Science Ltd, New York, NY, USA, 250: 110491, (2020).