Expression profiling of murine lung 70 days following exposure to fractionated or acute dose of 1.0 Gy 56Fe- particle irradiation
Irradiation of the K-rasLA1 mouse model with a fractionated dose of 1.0Gy 56Fe- particles increases the incidence of invasive carcinoma compared to unirradiated controls or those irradiated with an acute dose. Microarray profiling was perfromed on whole lungs from K-rasLA1 mice in order to determine global expression changes in the lung following radiation exposure. RNA was extracted from K-rasLA1 lungs from unirradiated control animals or those irradiated with a fractionated or acute dose of 1.0Gy 56Fe- particles 70 days post-irradiation when lungs are still histologically indistiguishable and only contain benign lesions. 129S2 K-rasLA1 mice were either irradiated with an acute (1.0Gy x1; n = 15) or fractionated (0.2Gy x5; n=13) dose of 1.0Gy 56Fe- particles or left unirradiated (n=13). Animals were euthanized 70 days post-irradiation and RNA was extracted from the whole left lung of each animal.
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Expression profiling of murine lung 70 days following exposure to fractionated or acute dose of 1.0 Gy 56Fe- particle irradiation
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| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[ "026:00" ] |
| contactPoint |
{ "fn": "GeneLab Outreach", "@type": "vcard:Contact", "hasEmail": "mailto:genelab-outreach@lists.nasa.gov" } |
| description | Irradiation of the K-rasLA1 mouse model with a fractionated dose of 1.0Gy 56Fe- particles increases the incidence of invasive carcinoma compared to unirradiated controls or those irradiated with an acute dose. Microarray profiling was perfromed on whole lungs from K-rasLA1 mice in order to determine global expression changes in the lung following radiation exposure. RNA was extracted from K-rasLA1 lungs from unirradiated control animals or those irradiated with a fractionated or acute dose of 1.0Gy 56Fe- particles 70 days post-irradiation when lungs are still histologically indistiguishable and only contain benign lesions. 129S2 K-rasLA1 mice were either irradiated with an acute (1.0Gy x1; n = 15) or fractionated (0.2Gy x5; n=13) dose of 1.0Gy 56Fe- particles or left unirradiated (n=13). Animals were euthanized 70 days post-irradiation and RNA was extracted from the whole left lung of each animal. |
| distribution |
[ { "@type": "dcat:Distribution", "title": "Expression profiling of murine lung 70 days following exposure to fractionated or acute dose of 1.0 Gy 56Fe- particle irradiation", "format": "HTML", "mediaType": "text/html", "description": "GeneLab Study Page", "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-148" } ] |
| identifier | nasa_genelab_GLDS-148_vy43-qjx6 |
| issued | 2018-06-26 |
| keyword |
[ "array-scanning-protocol", "fractionated-dose", "growth-protocol", "hybridization-protocol", "ionizing-radiation", "labelling-protocol", "normalization-data-transformation-protocol", "nucleic-acid-extraction-protocol", "p-gse42233-1", "p-gse42233-2", "p-gse42233-3", "p-gse42233-4", "p-gse42233-5", "p-gse42233-6", "p-gse42233-7", "sample-treatment-protocol", "sex" ] |
| landingPage | https://data.nasa.gov/dataset/expression-profiling-of-murine-lung-70-days-following-exposure-to-fractionated-or-acute-do |
| modified | 2025-04-23 |
| programCode |
[ "026:005" ] |
| publisher |
{ "name": "National Aeronautics and Space Administration", "@type": "org:Organization" } |
| theme |
[ "Earth Science" ] |
| title | Expression profiling of murine lung 70 days following exposure to fractionated or acute dose of 1.0 Gy 56Fe- particle irradiation |
