Expressed Sequence Tags from the Ciliate Protozoan Parasite Ichthyophthirius Multifiliis

Metadata Updated: February 4, 2022

Researchers sequenced 10,368 expressed sequence tags (EST) clones using a normalized cDNA library made from pooled samples of the trophont, tomont, and theront life-cycle stages, and generated 9,769 sequences (94.2% success rate). Post-sequencing processing led to 8,432 high quality sequences. Clustering analysis of these ESTs allowed identification of 4,706 unique sequences containing 976 contigs and 3,730 singletons. The ciliate protozoan Ichthyophthirius multifiliis (Ich) is an important parasite of freshwater fish that causes 'white spot disease' leading to significant losses. A genomic resource for large-scale studies of this parasite has been lacking. To study gene expression involved in Ich pathogenesis and virulence, our goal was to generate ESTs for the development of a powerful microarray platform for the analysis of global gene expression in this species. Here, we initiated a project to sequence and analyze over 10,000 ESTs.

Access & Use Information

Public: This dataset is intended for public access and use. License: Creative Commons CCZero

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IchthyophthiriusESTs_DataDictionary_0.csv
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Dates

Metadata Created Date	November 10, 2020
Metadata Updated Date	February 4, 2022

Metadata Source

Data.json Data.json Metadata
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Harvested from USDA JSON

Additional Metadata

Resource Type	Dataset
Metadata Created Date	November 10, 2020
Metadata Updated Date	February 4, 2022
Publisher	Agricultural Research Service
Maintainer	Liu, Zhanjiang
Identifier	5cc7124c-29b3-423b-9456-699e5d508880
Data Last Modified	2021-10-29
Public Access Level	public
Bureau Code	005:18
Metadata Context	https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Schema Version	https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby	https://project-open-data.cio.gov/v1.1/schema/catalog.json
Data Dictionary	https://data.nal.usda.gov/dataset/expressed-sequence-tags-ciliate-protozoan-parasite-ichthyophthirius-multifiliis/resource-2
Harvest Object Id	86628048-5407-46b6-b64d-8a04d4333b73
Harvest Source Id	d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
Harvest Source Title	USDA JSON
License	https://creativecommons.org/publicdomain/zero/1.0/
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Program Code	005:040
Source Datajson Identifier	True
Source Hash	be34d454e7dcca62fa1a3fdd61586201ffa99adf
Source Schema Version	1.1
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