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Expressed Sequence Tags from the Ciliate Protozoan Parasite Ichthyophthirius Multifiliis

Metadata Updated: February 4, 2022

Researchers sequenced 10,368 expressed sequence tags (EST) clones using a normalized cDNA library made from pooled samples of the trophont, tomont, and theront life-cycle stages, and generated 9,769 sequences (94.2% success rate). Post-sequencing processing led to 8,432 high quality sequences. Clustering analysis of these ESTs allowed identification of 4,706 unique sequences containing 976 contigs and 3,730 singletons. The ciliate protozoan Ichthyophthirius multifiliis (Ich) is an important parasite of freshwater fish that causes 'white spot disease' leading to significant losses. A genomic resource for large-scale studies of this parasite has been lacking. To study gene expression involved in Ich pathogenesis and virulence, our goal was to generate ESTs for the development of a powerful microarray platform for the analysis of global gene expression in this species. Here, we initiated a project to sequence and analyze over 10,000 ESTs.

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Public: This dataset is intended for public access and use. License: Creative Commons CCZero

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Metadata Created Date November 10, 2020
Metadata Updated Date February 4, 2022

Metadata Source

Harvested from USDA JSON

Additional Metadata

Resource Type Dataset
Metadata Created Date November 10, 2020
Metadata Updated Date February 4, 2022
Publisher Agricultural Research Service
Identifier 5cc7124c-29b3-423b-9456-699e5d508880
Data Last Modified 2021-10-29
Public Access Level public
Bureau Code 005:18
Metadata Context
Schema Version
Catalog Describedby
Data Dictionary
Harvest Object Id 86628048-5407-46b6-b64d-8a04d4333b73
Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
Harvest Source Title USDA JSON
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Program Code 005:040
Source Datajson Identifier True
Source Hash be34d454e7dcca62fa1a3fdd61586201ffa99adf
Source Schema Version 1.1
Spatial {"type":"Polygon","coordinates":-169.21874284744,-82.774222432791,-169.21874284744,84.73193488683,196.40625715256,84.73193488683,196.40625715256,-82.774222432791}

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