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Effect of PFAS Exposure on American Kestrels, Eastern Ecological Science Center, 2024

Published by U.S. Geological Survey | Department of the Interior | Catalog Last Checked: August 01, 2026 at 03:41 AM | Dataset Last Updated: February 05, 2026 at 12:00 AM
Serum samples were collected from an American Kestrel colony at the U.S. Geological Survey (USGS) Eastern Ecological Science Center and analyzed for per- and polyfluoroalkyl substances (PFAS). The sampling effort was undertaken as part of a Strategic Environmental Research and Development Program (SERDP) funded project (ER22-3388) to study the exposure effect of PFAS on immunomodulation in American Kestrels. Samples were collected in 2 milliliter cryogenic vials, stored at -80 degrees C, shipped to the USGS PFAS analytical laboratory on ice packs, and frozen at -80 degrees C until analysis. At the USGS PFAS analytical laboratory, samples were prepared with a direct injection method and analyzed with a Thermo Fisher Scientific™ Orbitrap Exploris™ 120 high resolution mass spectrometer coupled to a Vanquish Flex liquid chromatograph. This data release documents the sample preparation and analytical method.

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