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Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor

Published by National Institute of Standards and Technology | National Institute of Standards and Technology | Catalog Last Checked: August 02, 2025 at 02:26 PM | Dataset Last Updated: February 17, 2021
This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F, G2F, and SAF, in isolation and in complexation with the high-affinity receptor, Fc(gamma)RIa (CD64). The IgG1 antibody used in this study, aIL8hFc, is a murine-human chimeric IgG1, which inhibits IL-8 binding to human neutrophils. The working datasets comprise 17,750 deuterium uptake measurements in peptides from the receptor and the light and heavy chains of aIL8hFc obtained during 7 experiments, each comprising three runs of six exchange times. The data illuminate on the hydrogen exchange dynamics in isolated receptor, isolated IgG1 glycoforms, and receptor-IgG1 complexes.

Resources

37 resources available

  • DOI Access for Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor

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  • Glossary of Protein Modifications

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  • HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform.

    CSV
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  • HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform.

    CSV
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  • HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform.

    CSV
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  • HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.

    CSV
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  • HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.

    CSV
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  • HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.

    CSV
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  • HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a .

    CSV
    Download

  • HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.

    CSV
    Download

  • HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.

    CSV
    Download

  • HDX-MS data of peptides from isolated (apo-)Fc?R1a.

    CSV
    Download

  • HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc.

    CSV
    Download

  • HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc.

    CSV
    Download

  • HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc.

    CSV
    Download

  • HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc

    CSV
    Download

  • HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc

    CSV
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  • SHA256 File HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc

    TEXT/PLAIN
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  • HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc

    CSV
    Download

  • SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.

    TEXT/PLAIN
    Download

  • Sequences of aILhFc and soluble FcgR1a (CD64a)

    APPLICATION/PDF
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  • SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data of peptides from isolated (apo-)Fc(gamma)R1a.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a .

    TEXT/CSV
    Download

  • SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc

    CSV
    Download

  • SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc.

    CSV
    Download

  • SHA256 File for Glossary of Protein Modifications

    TEXT/PLAIN
    Download

  • SHA256 File for Sequences of aILhFc and soluble FcgR1a (CD64a)

    TEXT/PLAIN
    Download

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Complete Metadata
@type dcat:Dataset
accessLevel public
bureauCode 
[
  "006:55"
]
contactPoint 
{
  "fn": "Jeffrey W. Hudgens",
  "hasEmail": "mailto:jeffrey.hudgens@nist.gov"
}
description This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F, G2F, and SAF, in isolation and in complexation with the high-affinity receptor, Fc(gamma)RIa (CD64). The IgG1 antibody used in this study, aIL8hFc, is a murine-human chimeric IgG1, which inhibits IL-8 binding to human neutrophils. The working datasets comprise 17,750 deuterium uptake measurements in peptides from the receptor and the light and heavy chains of aIL8hFc obtained during 7 experiments, each comprising three runs of six exchange times. The data illuminate on the hydrogen exchange dynamics in isolated receptor, isolated IgG1 glycoforms, and receptor-IgG1 complexes.
distribution 
[
  {
    "title": "DOI Access for Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor",
    "accessURL": "https://doi.org/10.18434/mds2-2365"
  },
  {
    "title": "Glossary of Protein Modifications",
    "mediaType": "application/pdf",
    "description": "Glossary of post translational modifications present on peptides.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Glossary%20of%20Protein%20Modifications.pdf"
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  {
    "title": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform.",
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  },
  {
    "title": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2315_holo-FcgR1a%28G2F%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2316_holo-FcgR1a%28SAF%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%238_holo-G0F_heavy_chain%28FcgR1a%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2310_holo-G2F_heavy_chain%28FcgR1a%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2312_holo-SAF_heavy_chain%28FcgR1a%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a .",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%232_holo-G0F_light_chain%28FcgR1a%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%234_holo-G2F_light_chain%28FcgR1a%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%236_holo-SAF_light_chain%28FcgR1a%29.CSV"
  },
  {
    "title": "HDX-MS data of peptides from isolated (apo-)Fc?R1a.",
    "format": "CSV",
    "mediaType": "application/vnd.ms-excel",
    "description": "HDX-MS data of peptides from isolated (apo-)Fc?R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2313_apo-FcgR1a.CSV"
  },
  {
    "title": "HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%237_apo-G0F_heavy_chain.CSV"
  },
  {
    "title": "HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%239_apo-G2F_heavy_chain.CSV"
  },
  {
    "title": "HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2311_apo-SAF_heavy_chain.CSV"
  },
  {
    "title": "HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc",
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  },
  {
    "title": "HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc",
    "format": "csv",
    "mediaType": "text/csv",
    "description": "HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%233_apo-G2F_light_chain.CSV"
  },
  {
    "title": "SHA256 File HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc",
    "mediaType": "text/plain",
    "description": "HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%233_apo-G2F_light_chain.CSV.sha256"
  },
  {
    "title": "HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%235_apo-SAF_light_chain.CSV"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
    "mediaType": "text/plain",
    "description": "SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%236_holo-SAF_light_chain%28FcgR1a%29.CSV.sha256"
  },
  {
    "title": "Sequences of aILhFc and soluble FcgR1a (CD64a)",
    "mediaType": "application/pdf",
    "description": "Sequences of aILhFc and soluble FcgR1a (CD64a)",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Sequences%20of%20aILhFc%20and%20soluble%20FcgR1a%20%28CD64a%29.pdf"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2310_holo-G2F_heavy_chain%28FcgR1a%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2311_apo-SAF_heavy_chain.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2312_holo-SAF_heavy_chain%28FcgR1a%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data of peptides from isolated (apo-)Fc(gamma)R1a.",
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    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2313_apo-FcgR1a.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2314_holo-FcgR1a%28G0F%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2315_holo-FcgR1a%28G2F%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2316_holo-FcgR1a%28SAF%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%231_apo-G0F_light_chain.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a .",
    "mediaType": "text/csv",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%232_holo-G0F_light_chain%28FcgR1a%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%234_holo-G2F_light_chain%28FcgR1a%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc",
    "format": "CSV",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%235_apo-SAF_light_chain.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%237_apo-G0F_heavy_chain.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%238_holo-G0F_heavy_chain%28FcgR1a%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc.",
    "format": "CSV",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%239_apo-G2F_heavy_chain.CSV.sha256"
  },
  {
    "title": "SHA256 File for Glossary of Protein Modifications",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Glossary%20of%20Protein%20Modifications.pdf.sha256"
  },
  {
    "title": "SHA256 File for Sequences of aILhFc and soluble FcgR1a (CD64a)",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Sequences%20of%20aILhFc%20and%20soluble%20FcgR1a%20%28CD64a%29.pdf.sha256"
  }
]
identifier ark:/88434/mds2-2365
issued 2021-06-03
keyword 
[
  "HDX-MS",
  "antibody-receptor interaction",
  "chromatography",
  "glycosylation",
  "hydrogen-deuterium exchange",
  "mass spectrometry",
  "monoclonal antibody",
  "peptide",
  "precision",
  "protein",
  "proteolysis",
  "proteomics",
  "receptor."
]
language 
[
  "en"
]
license https://www.nist.gov/open/license
modified 2021-02-17 00:00:00
programCode 
[
  "006:045"
]
publisher 
{
  "name": "National Institute of Standards and Technology",
  "@type": "org:Organization"
}
theme 
[
  "Bioscience:Biomaterials",
  "Bioscience:Proteomics",
  "Chemistry:Analytical chemistry",
  "Chemistry:Molecular characterization",
  "Health:Pharmaceuticals",
  "Manufacturing:Biomanufacturing",
  "Materials:Materials characterization"
]
title Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor

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