Data from: Ploidy determination of buffel grass accessions in the USDA National Plant Germplasm System collection by flow cytometry

Metadata Updated: November 10, 2020

Buffel grass (Cenchrus ciliaris L. syn. Pennisetum ciliare (L.) Link) is an economically important warm-season perennial used primarily as a forage and range grass in many of the drier regions throughout the subtropics and tropics. Because buffel grass has excellent drought tolerance combined with desirable forage traits, it is an important forage and range grass in parts of Africa, including South Africa. It was introduced into many of the arid subtropical regions of the world, primarily Australia, North America, and South America, and today the species is grown on more than 50 million ha worldwide. Knowledge of the ploidy, meiotic behavior, and fertility of the plants used in hybridization programs became important because success depended upon using meiotically stable parental lines with compatible chromosome numbers. Little is known about the chromosome number of the buffel grass accessions in the USDA National Plant Germplasm System (NPGS) because the species has small chromosomes, that makes them difficult to count using traditional cytological methods. The DNA content of 568 accessions of buffel grass in the USDA National Plant Germplasm System was determined through flow cytometry to predict their ploidy levels. Based on DNA content, 308 accessions were determined as tetraploids with 36 chromosomes, 139 as pentaploids with 45 chromosomes, 20 as hexaploids with 54 chromosomes, two as septaploids with 63 chromosomes, and 99 as aneuploids. Chromosome counts of selected plants confirmed ploidy levels from DNA findings. Chromosome number of euploid plants could be predicted from the DNA data. The findings from this investigation reveal that the accessions in the buffel grass collection consist of a wide range of cytotypes, including polyploids and aneuploids with varying chromosome numbers. This demonstrates the amount of chromosomal diversity that occurs within the species and this collection. Grass breeders who use the buffel grass germplasm in the NPGS in their breeding programs should ensure that they know the chromosome number of the material requested. The predicted ploidy levels in Supplementary Table 1 will provide that information to the breeders.

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Public: This dataset is intended for public access and use. License: Creative Commons CCZero

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Dates

Metadata Created Date November 10, 2020
Metadata Updated Date November 10, 2020

Metadata Source

Harvested from USDA JSON

Additional Metadata

Resource Type Dataset
Metadata Created Date November 10, 2020
Metadata Updated Date November 10, 2020
Publisher Agricultural Research Service
Unique Identifier Unknown
Maintainer
Identifier d57b1a4d-a489-4196-bf98-9ed1d79784d5
Data Last Modified 2019-08-05
Public Access Level public
Bureau Code 005:18
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Data Dictionary https://data.nal.usda.gov/dataset/data-ploidy-determination-buffel-grass-accessions-usda-national-plant-germplasm-system-collection-flow-cytometry/resource/d5457330-047a-4e78-9ae2-4dc11f15976b
Harvest Object Id ea66bcec-3a7b-4490-9b0e-2d97a3d662c9
Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
Harvest Source Title USDA JSON
License https://creativecommons.org/publicdomain/zero/1.0/
Program Code 005:040
Source Datajson Identifier True
Source Hash 9b2dae6301c739203796517e32682a1bbe4bb63d
Source Schema Version 1.1

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