Return to search results
Data from: Perfluorooctanoic Acid (PFOA) Alters the Structure of the Gut Microbial Community and Colonoid Transcription
An ex vivo study of the effects of perfluorooctanoic acid (PFOA) on the human gut microbiota by testing the addition of PFOA to fecal incubations at a concentration of 20 g/L. Incubations were performed on fecal slurries from 18 donors spanning 3 age groups (adults 25-35 years, adults 35-50 years, and adults 50-70 years). Samples include initial time point (pre-incubation) and both untreated controls and treated samples after 48 hours of incubation. Data collected include: shotgun metagenomic DNA sequencing, biomass (cell counts), short-chain fatty acid concentrations, gas and pH. Colonoids were incubated with bacteria-free supernatants from this experiment and RNA-Seq performed. RNA-Seq counts data is provided in this dataset. These measures and donor metadata are in this dataset with sample IDs linking to sequencing data housed in the NCBI Sequence Read Archive associated with BioProject PRJNA1456031.The READ_ME.txt file describes each data file and columns/variables.
Resources
4 resources available
-
PFOA_metadata.csv
CSV -
READ_ME.txt
TXT -
PFOA_variance_stabliized_genes.csv
CSV -
PFOA_normalized_counts_transcripts_BFS_TPM.csv
CSV
Find Related Datasets
Search by Tags
Click any tag below to search for similar datasets
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "005:18" ] |
| contactPoint |
{ "fn": "Narrowe, Adrienne B.", "hasEmail": "mailto:adrienne.narrowe@usda.gov" } |
| description | <p dir="ltr">An<i> </i><i>ex vivo</i> study of the effects of perfluorooctanoic acid (PFOA) on the human gut microbiota by testing the addition of PFOA to fecal incubations at a concentration of 20 g/L. Incubations were performed on fecal slurries from 18 donors spanning 3 age groups (adults 25-35 years, adults 35-50 years, and adults 50-70 years). Samples include initial time point (pre-incubation) and both untreated controls and treated samples after 48 hours of incubation. Data collected include: shotgun metagenomic DNA sequencing, biomass (cell counts), short-chain fatty acid concentrations, gas and pH. Colonoids were incubated with bacteria-free supernatants from this experiment and RNA-Seq performed. RNA-Seq counts data is provided in this dataset. These measures and donor metadata are in this dataset with sample IDs linking to sequencing data housed in the NCBI Sequence Read Archive associated with BioProject PRJNA1456031.</p><p dir="ltr">The READ_ME.txt file describes each data file and columns/variables.</p><p dir="ltr"><br></p> |
| distribution |
[ { "@type": "dcat:Distribution", "title": "PFOA_metadata.csv", "format": "csv", "mediaType": "text/csv", "downloadURL": "https://ndownloader.figshare.com/files/65286912" }, { "@type": "dcat:Distribution", "title": "READ_ME.txt", "format": "txt", "mediaType": "text/plain", "downloadURL": "https://ndownloader.figshare.com/files/65407647" }, { "@type": "dcat:Distribution", "title": "PFOA_variance_stabliized_genes.csv", "format": "csv", "mediaType": "text/csv", "downloadURL": "https://ndownloader.figshare.com/files/65407653" }, { "@type": "dcat:Distribution", "title": "PFOA_normalized_counts_transcripts_BFS_TPM.csv", "format": "csv", "mediaType": "text/csv", "downloadURL": "https://ndownloader.figshare.com/files/65407668" } ] |
| identifier | 10.15482/USDA.ADC/32424846.v1 |
| keyword |
[ "PFOA", "Short chain fatty acids (SCFAs)", "colonoid", "gut microbiome", "perfluoroctanoic acid", "transcription" ] |
| license | https://creativecommons.org/publicdomain/zero/1.0/ |
| modified | 2026-06-26 |
| programCode |
[ "005:040" ] |
| publisher |
{ "name": "Agricultural Research Service", "@type": "org:Organization" } |
| temporal | 2023-10-03/2025-06-28 |
| title | Data from: Perfluorooctanoic Acid (PFOA) Alters the Structure of the Gut Microbial Community and Colonoid Transcription |
