Data from: Multiple, low-dose copper sulfate (CuSO<sub>4</sub>·5H<sub>2</sub>O) treatment effects on water quality and bacterial, phytoplankton, and zooplankton communities in catfish aquaculture ponds
In US catfish aquaculture, copper sulfate pentahydrate (CSP; CuSO4·5H2O) is used for a wide variety of applications including the control of blue-green algae, pathogenic bacteria, and parasite hosts. However, information on the pond-wide effects of multiple low-dose treatments is lacking. Herein, the influence of repeated, low CSP doses on water quality, phytoplankton, zooplankton, microbial populations, and fish production were evaluated.In March 2022, sixteen experimental ponds (0.31 ha) were stocked with Channel Catfish (Ictalurus punctatus; 8,000 fish/pond) and, beginning July 21 were treated with CSP doses for 4 consecutive weeks. Samples were collected before the first treatment, then taken weekly until harvest on October 13, 2022. Chlorophyll-a, total ammonia-N, nitrite-N, total oxidized nitrogen and nitrate-N were determined, major phytoplankton taxa identified, zooplankton communities assessed, and microbiota evaluated by 16S rRNA gene (V4 region) sequencing (available at NCBI). Individual ponds were used as replicates for all analyses (n = 4 per treatment). Treatment groups included non-treated controls ("Control" or "C" in the dataset), 0.5 mg/L CSP ("Low" or "L"), 1.0 mg/L CSP ("Medium" or "M"), and 1.5 mg/L CSP ("High" or "H").At each sampling period, 50 mL of water were collected approximately 2.5 cm below the water surface to evaluate water quality, phytoplankton, and zooplankton. Chlorophyll-a was measuring using a chloroform-methanol extraction. Free copper (cupric ions), alkalinity, hardness, total ammonia-N, nitrite-N, nitrate-N, and total oxidized nitrogen were measured using a Gallery Discrete Analyzer and the automated protocols provided by the manufacturer.For phytoplankton, samples were preserved in 10% Lugol's solution. One mL of solution was placed on a Sedgewick-Rafter counting cell and organisms within 10 fields of view were counted using a light microscope at 400x magnification. Abundance is recorded in natural units (i.e., unialgal cells, colonies, or filaments).Zooplankton were collected using a 2-m oblique tow with an 80µm Wisconsin-style plankton net. Samples were concentrated to 240 mL and preserved in 10% buffered formalin solution. All organisms from three 1-mL subsamples per pond were identified and counted using a Sedgewick-Rafter counting cell.All analyses were performed using program R (v4.5.0) with the RStudio interface (v2025.5.0.496).
Resources
4 resources available
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copper_pond_production.csv
CSV -
pond_copper_phyto_genus.csv
CSV -
pond_copper_wq.csv
CSV -
pond_copper_zoops.csv
CSV
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | R/P1W |
| bureauCode |
[ "005:18" ] |
| contactPoint |
{ "fn": "Richardson, Bradley M.", "hasEmail": "mailto:brad.richardson@usda.gov" } |
| description | <p dir="ltr">In US catfish aquaculture, copper sulfate pentahydrate (CSP; CuSO<sub>4</sub>·5H<sub>2</sub>O) is used for a wide variety of applications including the control of blue-green algae, pathogenic bacteria, and parasite hosts. However, information on the pond-wide effects of multiple low-dose treatments is lacking. Herein, the influence of repeated, low CSP doses on water quality, phytoplankton, zooplankton, microbial populations, and fish production were evaluated.</p><p dir="ltr">In March 2022, sixteen experimental ponds (0.31 ha) were stocked with Channel Catfish (<i>Ictalurus punctatus</i>; 8,000 fish/pond) and, beginning July 21 were treated with CSP doses for 4 consecutive weeks. Samples were collected before the first treatment, then taken weekly until harvest on October 13, 2022. Chlorophyll-<i>a</i>, total ammonia-N, nitrite-N, total oxidized nitrogen and nitrate-N were determined, major phytoplankton taxa identified, zooplankton communities assessed, and microbiota evaluated by 16S rRNA gene (V4 region) sequencing (available at NCBI). Individual ponds were used as replicates for all analyses (n = 4 per treatment). Treatment groups included non-treated controls ("Control" or "C" in the dataset), 0.5 mg/L CSP ("Low" or "L"), 1.0 mg/L CSP ("Medium" or "M"), and 1.5 mg/L CSP ("High" or "H").</p><p dir="ltr">At each sampling period, 50 mL of water were collected approximately 2.5 cm below the water surface to evaluate water quality, phytoplankton, and zooplankton. Chlorophyll-<i>a</i> was measuring using a chloroform-methanol extraction. Free copper (cupric ions), alkalinity, hardness, total ammonia-N, nitrite-N, nitrate-N, and total oxidized nitrogen were measured using a Gallery Discrete Analyzer and the automated protocols provided by the manufacturer.</p><p dir="ltr">For phytoplankton, samples were preserved in 10% Lugol's solution. One mL of solution was placed on a Sedgewick-Rafter counting cell and organisms within 10 fields of view were counted using a light microscope at 400x magnification. Abundance is recorded in natural units (i.e., unialgal cells, colonies, or filaments).</p><p dir="ltr">Zooplankton were collected using a 2-m oblique tow with an 80µm Wisconsin-style plankton net. Samples were concentrated to 240 mL and preserved in 10% buffered formalin solution. All organisms from three 1-mL subsamples per pond were identified and counted using a Sedgewick-Rafter counting cell.</p><p dir="ltr">All analyses were performed using program R (v4.5.0) with the RStudio interface (v2025.5.0.496).</p> |
| distribution |
[ { "@type": "dcat:Distribution", "title": "copper_pond_production.csv", "format": "csv", "mediaType": "text/csv", "downloadURL": "https://ndownloader.figshare.com/files/66121235" }, { "@type": "dcat:Distribution", "title": "pond_copper_phyto_genus.csv", "format": "csv", "mediaType": "text/csv", "downloadURL": "https://ndownloader.figshare.com/files/66121238" }, { "@type": "dcat:Distribution", "title": "pond_copper_wq.csv", "format": "csv", "mediaType": "text/csv", "downloadURL": "https://ndownloader.figshare.com/files/66121241" }, { "@type": "dcat:Distribution", "title": "pond_copper_zoops.csv", "format": "csv", "mediaType": "text/csv", "downloadURL": "https://ndownloader.figshare.com/files/66121244" } ] |
| identifier | 10.15482/USDA.ADC/32840378.v1 |
| keyword |
[ "aquaculture", "channel catfish (Ictalurus punctatus)", "copper sulfate pentahydrate", "microbiome", "phytoplankton", "zooplankton" ] |
| license | https://creativecommons.org/publicdomain/zero/1.0/ |
| modified | 2026-07-06 |
| publisher |
{ "name": "Agricultural Research Service", "@type": "org:Organization" } |
| spatial |
"{"type": "Polygon", "coordinates": [[[-90.90669052535708, 33.442660879178206], [-90.89050867689693, 33.442660879178206], [-90.89050867689693, 33.45307861570761], [-90.90669052535708, 33.45307861570761], [-90.90669052535708, 33.442660879178206]]]}"
|
| temporal | 2022-07-21/2022-10-13 |
| title | Data from: Multiple, low-dose copper sulfate (CuSO<sub>4</sub>·5H<sub>2</sub>O) treatment effects on water quality and bacterial, phytoplankton, and zooplankton communities in catfish aquaculture ponds |
