Data from: Monitoring the impact of chlorinated drinking water on the developing gut microbiota of adolescent mice using genetic profiling

For over a century, water chlorination as a method to deter growth of disease-causing microbes has been standard practice in the United States. Original toxicological studies designated chlorinated water as safe for the human host but did not consider the potential impact of chlorine on the gut microbiota, particularly the developing gut microbiota post-weaning. Here, a comparative assessment of the gut microbiota from post-weaned mice (B6) over the course of four weeks provided either chlorine-free water or water containing 1-5 mg/L chlorine was performed. Male and female B6 mice (F0) were provided Deer Park spring water and purified Diet Pellets made with Deer Park spring water and used to breed chlorine-free pups (F1). F2 pups were weaned at 21 days and divided into the following groups: 1) chlorine free, which were continued on the chlorine free diet and 2) chlorine treated, which were provided Deer Park spring water with 5mg/L chlorine added. Fecal pellets were harvested from each cage every week for 4 weeks and 16S rRNA sequencing of the V1-V2 region was performed to determine community structure. SCFA analysis was performed to determine community function.

[
  "005:18"
]

{
  "fn": "Narrowe, Adrienne B.",
  "hasEmail": "mailto:adrienne.narrowe@usda.gov"
}

[
  {
    "@type": "dcat:Distribution",
    "title": "chlorine_metadata.csv",
    "format": "csv",
    "mediaType": "text/csv",
    "downloadURL": "https://ndownloader.figshare.com/files/65288982"
  }
]

[
  "16S r RNA sequencing",
  "adenosylcobalamin",
  "chlorination",
  "microbiome",
  "vitamin b12",
  "water"
]

[
  "005:040"
]

{
  "name": "Agricultural Research Service",
  "@type": "org:Organization"
}