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Data from: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks
This study produced a comprehensive transcriptome from newly molted adult ticks and will provide a useful resource for studies of tick feeding and host perception and also assist genome annotation refinements.
The goal was to develop a comprehensive transcriptome of adult female Rhipicephalus microplus ticks and discover transcriptome changes that are related to feeding and host-perception prior to feeding. We have sequenced the transcriptomes of unfed newly molted adult female ticks, newly molted adult females that were feeding upon a bovine host for 3 hours following attachment to the host, and newly molted adult females that were placed in a gas-permeable bag attached to a bovine host for 3 hours (attachment to host prevented but host odors available to the ticks). There were 2 biological replicates and transcriptomes assembled from each individual experimental condition. A collective adult female transcriptome was assembled from sequence data pooled from all 3 conditions (using data from all replicates). Differential gene expression was examined using all possible comparisons to detect putative feeding-associated and host perception-associated transcripts. Resources in this dataset:Resource Title: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks. File Name: Web Page, url: https://doi.org/10.1007/s10493-019-00420-1
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "005:18" ] |
| contactPoint |
{ "fn": "Bendele, Kylie", "hasEmail": "mailto:Kylie.Bendele@usda.gov" } |
| description | <p>This study produced a comprehensive transcriptome from newly molted adult ticks and will provide a useful resource for studies of tick feeding and host perception and also assist genome annotation refinements.</p> <p>The goal was to develop a comprehensive transcriptome of adult female Rhipicephalus microplus ticks and discover transcriptome changes that are related to feeding and host-perception prior to feeding. We have sequenced the transcriptomes of unfed newly molted adult female ticks, newly molted adult females that were feeding upon a bovine host for 3 hours following attachment to the host, and newly molted adult females that were placed in a gas-permeable bag attached to a bovine host for 3 hours (attachment to host prevented but host odors available to the ticks). There were 2 biological replicates and transcriptomes assembled from each individual experimental condition. A collective adult female transcriptome was assembled from sequence data pooled from all 3 conditions (using data from all replicates). Differential gene expression was examined using all possible comparisons to detect putative feeding-associated and host perception-associated transcripts. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks.</p> <p>File Name: Web Page, url: <a href="https://doi.org/10.1007/s10493-019-00420-1">https://doi.org/10.1007/s10493-019-00420-1</a> </p></li></ul><p></p> |
| distribution |
[ { "@type": "dcat:Distribution", "title": "https://doi.org/10.1007/s10493-019-00420-1", "mediaType": "text/html", "downloadURL": "https://doi.org/10.1007/s10493-019-00420-1" } ] |
| identifier | 10.1007/s10493-019-00420-1 |
| keyword |
[ "ARS", "NP104", "cattle tick", "data.gov", "feeding response", "gene expression", "host perception", "transcriptomics" ] |
| license | https://creativecommons.org/publicdomain/zero/1.0/ |
| modified | 2025-11-21 |
| programCode |
[ "005:040" ] |
| publisher |
{ "name": "Agricultural Research Service", "@type": "org:Organization" } |
| spatial |
"{"type": "Point", "coordinates": [-98.32857, 26.38684]}"
|
| temporal | 2008-11-17/2008-11-18 |
| title | Data from: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks |