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Data from: Fructooligosaccharides (FOS) differentially modifies the in vitro gut microbiota in an age-dependent manner

Published by Agricultural Research Service | Department of Agriculture | Catalog Last Checked: May 05, 2026 at 11:40 PM | Dataset Last Updated: November 21, 2025
A 24-h in vitro culturing method was used to determine whether fructooligosaccharide (FOS) treatment effects on the human gut microbiome has a differential effect by age, using fecal samples from 18 adults in 3 age groups with 6 individuals for each group, young adult (25–35 years of age), adult (36–50 years of age), and older adult (51–70 years of age). Data collected include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (available in the NCBI Sequence Read Archive associated with BioProject PRJNA883714: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883714), total bacterial qPCR, Bifidobacterium genus specific q-PCR, and short chain fatty acid concentrations. Resources in this dataset: Resource Title: Sample metadata File Name: FOS_sample_attributes.csv Resource Title: Short Chain Fatty Acid concentrations File Name: FOS_SCFA_data.csv Resource Title: Total bacterial and Bifidobacterium genus qPCR File Name: total_bacteria_and_Bifidobacterium_specific_qPCR.csv

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