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Data from: Attraction, mobility, and preference by Lasioderma serricorne (F.) (Coleoptera: Ptinidae) to microbially-mediated volatile emissions by two species of fungi in stored grain

Published by Agricultural Research Service | Department of Agriculture | Catalog Last Checked: May 05, 2026 at 11:47 PM | Dataset Last Updated: November 21, 2025
Our goals were to 1) isolate, and culture two fungal morphotypes, 2) characterize the volatile emissions from grain inoculated by each fungal morphotype (Aspergillus flavus or Fusarium spp.) compared to uninoculated and sanitized grain, and 3) understand how MVOCs from each morphotype affects mobility, attraction, and preference by L. serricorne. Headspace collection revealed that the Fusarium- and A. flavus-inoculated grain produced significantly different volatiles compared to sanitized grain or the positive control. Changes in MVOC emissions affected close-range foraging during an Ethovision assay, with a greater frequency of entering and spending time in a small zone with kernels inoculated with A. flavus compared to other treatments. In the release-recapture assay, MVOCs were found to be attractive to L. serricorne at a longer distances in commercial pitfall traps. While there was no preference shown among semiochemical stimuli in a still-air, four-way olfactometer, it is possible that methodological limitations prevented robust interpretation from this assay. Overall, our study suggests that MVOCs are important for close- and long-range orientation of L.serricorne during foraging, and that MVOCs may have the potential for inclusion in behaviorally-based tactics for this species. Resources in this dataset:Resource Title: 4-way olfactometer assay. File Name: Sierra_2021_olfactometer_Ag_Data_commons.csvResource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel Resource Title: Release-recapture Assay . File Name: sierra_release_recapture_exp_2021_fungal_volatiles_agdata_commons.csvResource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel Resource Title: Ethovision Movement Assay . File Name: ethovision_sierra_2021_microbial_volatiles_agdatacommons.csvResource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel Resource Title: Headspace volatile collection assay. File Name: headspace_compounds_sierra_2021_fungal_volatiles_final_agdatacommons.csvResource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel Resource Title: Sequencing Data. File Name: sequencing_data.zipResource Title: File list. File Name: File_list_L_serricone_attraction_data.txt

Resources

6 resources available

  • Sierra_2021_olfactometer_Ag_Data_commons.csv

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  • sierra_release_recapture_exp_2021_fungal_volatiles_agdata_commons.csv

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  • ethovision_sierra_2021_microbial_volatiles_agdatacommons.csv

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  • headspace_compounds_sierra_2021_fungal_volatiles_final_agdatacommons.csv

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  • sequencing_data.zip

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  • File_list_L_serricone_attraction_data.txt

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Complete Metadata
@type dcat:Dataset
accessLevel public
bureauCode 
[
  "005:18"
]
contactPoint 
{
  "fn": "Morrison, William R.",
  "hasEmail": "mailto:william.morrison@usda.gov"
}
description <p>Our goals were to 1) isolate, and culture two fungal morphotypes, 2) characterize the volatile emissions from grain inoculated by each fungal morphotype (<em>Aspergillus flavus</em> or <em>Fusarium</em> spp.) compared to uninoculated and sanitized grain, and 3) understand how MVOCs from each morphotype affects mobility, attraction, and preference by <em>L. serricorne</em>. Headspace collection revealed that the <em>Fusarium</em>- and <em>A. flavus</em>-inoculated grain produced significantly different volatiles compared to sanitized grain or the positive control. Changes in MVOC emissions affected close-range foraging during an Ethovision assay, with a greater frequency of entering and spending time in a small zone with kernels inoculated with <em>A. flavus</em> compared to other treatments. In the release-recapture assay, MVOCs were found to be attractive to <em>L. serricorne</em> at a longer distances in commercial pitfall traps. While there was no preference shown among semiochemical stimuli in a still-air, four-way olfactometer, it is possible that methodological limitations prevented robust interpretation from this assay. Overall, our study suggests that MVOCs are important for close- and long-range orientation of <em>L.serricorne</em> during foraging, and that MVOCs may have the potential for inclusion in behaviorally-based tactics for this species.</p> <div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: 4-way olfactometer assay.</p> <p>File Name: Sierra_2021_olfactometer_Ag_Data_commons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Release-recapture Assay .</p> <p>File Name: sierra_release_recapture_exp_2021_fungal_volatiles_agdata_commons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Ethovision Movement Assay .</p> <p>File Name: ethovision_sierra_2021_microbial_volatiles_agdatacommons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Headspace volatile collection assay.</p> <p>File Name: headspace_compounds_sierra_2021_fungal_volatiles_final_agdatacommons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Sequencing Data.</p> <p>File Name: sequencing_data.zip</p></li><br><li><p>Resource Title: File list.</p> <p>File Name: File_list_L_serricone_attraction_data.txt</p></li></ul>
distribution 
[
  {
    "@type": "dcat:Distribution",
    "title": "Sierra_2021_olfactometer_Ag_Data_commons.csv",
    "format": "csv",
    "mediaType": "application/csv",
    "downloadURL": "https://ndownloader.figshare.com/files/44530868"
  },
  {
    "@type": "dcat:Distribution",
    "title": "sierra_release_recapture_exp_2021_fungal_volatiles_agdata_commons.csv",
    "format": "csv",
    "mediaType": "text/plain",
    "downloadURL": "https://ndownloader.figshare.com/files/44530871"
  },
  {
    "@type": "dcat:Distribution",
    "title": "ethovision_sierra_2021_microbial_volatiles_agdatacommons.csv",
    "format": "csv",
    "mediaType": "application/csv",
    "downloadURL": "https://ndownloader.figshare.com/files/44530874"
  },
  {
    "@type": "dcat:Distribution",
    "title": "headspace_compounds_sierra_2021_fungal_volatiles_final_agdatacommons.csv",
    "format": "csv",
    "mediaType": "text/plain",
    "downloadURL": "https://ndownloader.figshare.com/files/44530877"
  },
  {
    "@type": "dcat:Distribution",
    "title": "sequencing_data.zip",
    "format": "zip",
    "mediaType": "application/zip",
    "downloadURL": "https://ndownloader.figshare.com/files/44530883"
  },
  {
    "@type": "dcat:Distribution",
    "title": "File_list_L_serricone_attraction_data.txt",
    "format": "txt",
    "mediaType": "text/plain",
    "downloadURL": "https://ndownloader.figshare.com/files/44530886"
  }
]
identifier 10.15482/USDA.ADC/1528422
keyword 
[
  "ARS",
  "Coleoptera",
  "IPM",
  "Insects",
  "MVOCs",
  "NP304",
  "behavioral ecology",
  "data.gov",
  "entomology",
  "microbes",
  "mobility",
  "olfaction",
  "stored products",
  "trapping",
  "volatile responses"
]
license https://creativecommons.org/publicdomain/zero/1.0/
modified 2025-11-21
programCode 
[
  "005:040"
]
publisher 
{
  "name": "Agricultural Research Service",
  "@type": "org:Organization"
}
spatial 
"{"type": "Point", "coordinates": [-96.599108576775, 39.196002370935]}"
temporal 2021-06-10/2021-08-27
title Data from: Attraction, mobility, and preference by Lasioderma serricorne (F.) (Coleoptera: Ptinidae) to microbially-mediated volatile emissions by two species of fungi in stored grain

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