Data from: An In Vitro Combined Antibiotic-Antibody Treatment Eliminates Toxicity from Shiga Toxin-Producing Escherichia coli

Metadata Updated: February 21, 2020

Treating Shiga toxin-producing Escherichia coli (STEC) gastrointestinal infections is difficult. The utility of antibiotics for STEC treatment is controversial, since antibiotic resistance among STEC isolates is widespread and certain antibiotics dramatically increase the expression of Shiga toxins (Stxs), which are some of the most important virulence factors in STEC. Stxs contribute to life-threatening hemolytic uremic syndrome (HUS), which develops in considerable proportions of patients with STEC infections. Understanding the antibiotic resistance profiles of STEC isolates and the Stx induction potential of promising antibiotics is essential for evaluating any antibiotic treatment of STEC. In this study, 42 O157:H7 or non-O157 STEC isolates (including the “big six” serotypes) were evaluated for their resistance against 22 antibiotics by using an antibiotic array. Tigecycline inhibited the growth of all of the tested STEC isolates and also inhibited the production of Stxs (Stx2 in particular). In combination with neutralizing antibodies to Stx1 and Stx2, the tigecycline-antibody treatment fully protected Vero cells from Stx toxicity, even when the STEC bacteria and the Vero cells were cultured together. The combination of an antibiotic such as tigecycline with neutralizing antibodies presents a promising strategy for future STEC treatments.

Access & Use Information

Public: This dataset is intended for public access and use. License: Creative Commons CCZero

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Dates

Metadata Created Date February 21, 2020
Metadata Updated Date February 21, 2020

Metadata Source

Harvested from USDA JSON

Additional Metadata

Resource Type Dataset
Metadata Created Date February 21, 2020
Metadata Updated Date February 21, 2020
Publisher Agricultural Research Service
Unique Identifier 8286064e-af7d-4c7d-92ef-ac2fe1d7a1ad
Maintainer
He, Xiaohua
Maintainer Email
Public Access Level public
Bureau Code 005:18
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id c7ce1902-5b13-4be0-aac3-8878e4b01e8c
Harvest Source Id 50ca39af-9ddb-466d-8cf3-84d67a204346
Harvest Source Title USDA JSON
License https://creativecommons.org/publicdomain/zero/1.0/
Data Last Modified 2019-11-26
Program Code 005:040
Source Datajson Identifier True
Source Hash 1b2e6ee2dcf6a277f3a2e37cb7dce172330ebacf
Source Schema Version 1.1

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